Charcoal stabilization of phenyl phosphates
First Claim
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1. A method for stabilizing a solubilized phenyl phosphate comprising contacting the solubilized phenyl phosphate with a stabilizing amount of charcoal.
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Abstract
The invention discloses methods, compositions and kits for stabilizing a solubilized phenyl phosphate, preferably paranitrophenyl phosphate (PNPP), using charcoal. Also disclosed are methods, compositions and kits for recycling solubilized phenyl phosphate, preferably PNPP, that has an absorbance of less than 0.1 when measured at 405 nm due to non-enzymatic hydrolysis.
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Citations
47 Claims
- 1. A method for stabilizing a solubilized phenyl phosphate comprising contacting the solubilized phenyl phosphate with a stabilizing amount of charcoal.
- 10. A kit for phosphatase-phenyl phosphate reactions comprising a phosphatase and a stabilized solubilized phenyl phosphate.
- 13. A method for recycling a solubilized phenyl phosphate comprising admixing colored, solubilized phenyl phosphate with a recycling amount of charcoal.
- 22. A stabilized, solubilized phenyl phosphate comprising a buffer, a phenyl phosphate, and a stabilizing amount of charcoal.
- 30. A ready-to-use enzyme substrate composition comprising phenyl phosphate, a buffer, and charcoal.
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38. A method of preparing an aqueous liquid substrate system used in phosphatase enzyme determination comprising:
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(a) solubilizing a phenyl phosphate in an aqueous buffered solvent to provide a phenyl phosphate solution;
(b) adding a magnesium compound to the phenyl phosphate solution;
(c) contacting the solution with a stabilizing amount of charcoal; and
(d) sealing the solution. - View Dependent Claims (39, 40)
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- 41. A vessel for containing a solubilized phenyl phosphate in a basic buffer, wherein the vessel comprises charcoal on the surface of the vessel exposed to the solubilized phenyl phosphate.
- 43. A kit for recycling a solubilized phenyl phosphate having an absorbance over 0.1 OD due to non-enzymatic hydrolysis when measured at 400 to 410 nm comprising a stabilizing amount of charcoal.
Specification