Enzyme activity measuring apparatus, enzyme activity measuring method, sample transcription apparatus and color measuring apparatus

• US 20050124060A1
• Filed: 10/18/2004
• Published: 06/09/2005
• Est. Priority Date: 10/20/2003
• Status: Abandoned Application

• Current Assignee: Nipro Corporation, Toyama University, Yamaha Hatsudoki Kabushiki Kaisha (Yamaha Corporation)
• Original Assignee: Nipro Corporation, Toyama University, Yamaha Hatsudoki Kabushiki Kaisha (Yamaha Corporation)
• Inventors: Matsuda, Atsushi, Suzuki, Kazuhito, Higashi, Tomoyuki, Yamaguchi, Masaki
• Application Number:
  US10/967,643
• Publication Number:
  US 20050124060A1
• Time in Patent Office: Days
• US Class Current: 435/287.900
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04

Abstract

An enzyme activity measuring apparatus comprises a reagent/sample disposing unit 5 which disposed a sample and a reagent containing member 13 including an enzyme activity measuring reagent at a distance from each other, a sample transcription unit 7 which brings the disposed reagent containing member 13 and the sample into contact with each other to transcribe a predetermined volume of sample to the reagent containing member 13, a color depth measuring unit 9 configured to measure color depth of a reagent included in the reagent containing member 13 when predetermined time is elapsed after the sample is transcribed, and CPU 101 configured to detect an activity of enzyme included in the sample based on the measured color depth.

Claims

1. An enzyme activity measuring apparatus comprising

a reagent/sample disposing unit configured to dispose a sample and a reagent containing member including an enzyme activity measuring reagent which measures an activity of an enzyme at a distance from each other,

a sample transcription unit configured to bring the sample and the reagent containing member disposed by the reagent/sample disposing unit into contact with each other, and to transcribe a predetermined volume of the sample to the reagent containing member,

a color depth measuring unit configured to measure a color depth of reagent included in the reagent containing member when predetermined time is elapsed after the sample is transcribed, and

an enzyme activity detector configured to detect the activity of enzyme included in the sample based on the color depth measured by the color depth measuring unit.

2. The enzyme activity measuring apparatus according to claim 1, wherein the reagent containing member is a sheet member,

the sample is included in a sample containing member which is a sheet member,

the reagent/sample disposing unit disposes a reagent containing member flat surface which is a flat surface portion of the reagent containing member and a sample containing member flat surface which is a flat surface portion of the sample containing member such that these flat surfaces are opposed to each other in substantially parallel to each other,

the sample transcription unit brings the reagent containing member flat surface and the sample containing member flat surface into contact with each other for predetermined time under predetermined pressure.

3. The enzyme activity measuring apparatus according to claim 2, wherein the reagent/sample disposing unit disposes the reagent containing member and the sample containing member at a distance from each other using a holder which holds the reagent containing member and the sample containing member,

the sample transcription unit pushes the holder to bring the reagent containing member flat surface and the sample containing member flat surface into contact with each other.

4. The enzyme activity measuring apparatus according to claim 3, further comprising a cover which covers the color depth measuring unit and which opens and closes around a turning shaft, wherein

the sample transcription unit is provided on the cover.

5. The enzyme activity measuring apparatus according to claim 4, further comprising a projecting member which moves in a direction substantially perpendicular to a pushing direction in which the sample transcription unit pushes the holder, wherein

the cover is provided with an insertion hole into which the projecting member is inserted in a state in which the cover is closed.

6. The enzyme activity measuring apparatus according to claim 5, wherein

the projecting member is biased in an insertion direction into the insertion hole,

the enzyme activity measuring apparatus further comprises a projecting member moving lever which is engaged with the projecting member and which moves the projecting member in a direction opposite from the insertion direction by turning the projecting member moving lever around the turning shaft, and

a cover opening button portion which pushes the projecting member moving lever and which turns the projecting member moving lever when the cover opening button portion is pushed down.

7. The enzyme activity measuring apparatus according to claim 4, wherein

the sample transcription unit comprises

a movable member which has a pushing surface for pushing the holder and a cam abutment surface which is opposed to the pushing surface, and which can move around the pushing direction in which the holder is pushed,

a cam having a flat surface portion on a portion of its outer peripheral surface in which a distance from the turning shaft to the outer peripheral surface is longer than that of other portion,

a transcription lever which brings the flat surface portion into abutment against the cam abutment surface by turning the transcription lever into a first direction,

a first biasing member configured to bias the cam such as to turn the cam in a second direction which is opposite from the first direction, and

a second biasing member configured to bias the movable member toward the cam.

8. The enzyme activity measuring apparatus according to claim 7, further comprising a third biasing member configured to bias the transcription lever such as to turn the transcription lever in the second direction.

9. The enzyme activity measuring apparatus according to claim 1, further comprising

a temperature detector configured to detect a sample temperature which is a temperature of the sample,

a sample temperature correcting data storing unit configured to store sample temperature correcting data in which the sample temperature and correcting rate for correcting the activity detected by the enzyme activity detector are associated with each other, and

an activity correcting unit configured to correct the activity based on the sample temperature and the sample temperature correcting data.

10. The enzyme activity measuring apparatus according to claim 9, further comprising

a heater configured to heat the sample to predetermined temperature, and

a heater controller configured to stop the operation of the heater when the temperature detector detects that the sample temperature reaches the predetermined temperature.

11. The enzyme activity measuring apparatus according to claim 1, wherein

the color depth measuring unit irradiates the reagent containing member flat surface with light substantially at right angles, the color depth is measured by measuring light reflected from the reagent containing member flat surface from a direction which is diagonal with respect to the reagent containing member flat surface.

12. The enzyme activity measuring apparatus according to claim 11, wherein the color depth measuring unit measures the reflected intensity of light by receiving the same through optical fiber.

13. The enzyme activity measuring apparatus according to claim 1, wherein

the enzyme is amylase, and

the sample is saliva.

14. An enzyme activity measuring method comprising

a reagent/sample disposing step for disposing a sample and a reagent containing member including an enzyme activity measuring reagent which measures an activity of an enzyme at a distance from each other,

a sample transcription step for bringing the sample and the reagent containing member disposed in the reagent/sample disposing step into contact with each other, and for transcribing a predetermined volume of the sample to the reagent containing member,

a color depth measuring step for measuring a color depth of reagent included in the reagent containing member when predetermined time is elapsed after the sample is transcribed, and

an enzyme activity detecting step for detecting the activity of enzyme included in the sample based on the color depth measured in the color depth measuring step, wherein

in the reagent/sample disposing step, a reagent containing member flat surface which is a flat surface portion of the reagent containing member and a sample containing member flat surface which is a flat surface portion of the sample containing member are disposed such that these flat surfaces are opposed to each other in substantially parallel to each other,

in the sample transcription step, the reagent containing member flat surface and the sample containing member flat surface are brought into contact with each other for predetermined time under predetermined pressure.

15. A sample transcription apparatus comprising

a reagent/sample disposing unit configured to dispose a sheet reagent containing member including enzyme activity measuring reagent which measures activity of enzyme and a sheet sample containing member including sample at a distance from each other, and

a sample transcription unit which bring the reagent containing member and the sample disposed by the reagent/sample disposing unit and which transcribes a predetermined volume of sample to the reagent containing member, wherein

the reagent/sample disposing unit disposes a reagent containing member flat surface which is a flat surface portion of the reagent containing member and a sample containing member flat surface which is a flat surface portion of the sample containing member such that these flat surfaces are opposed to each other in substantially parallel to each other,

the sample transcription unit brings the reagent containing member flat surface and the sample containing member flat surface into contact with each other for predetermined time under predetermined pressure.

16. A color measuring apparatus comprising a color depth measuring unit which irradiates a reagent containing member flat surface which is a flat surface portion of a sheet reagent containing member including an enzyme activity measuring reagent which measures activity of enzyme with light substantially at right angles, and which measures the light reflected from the reagent containing member flat surface from a direction which is diagonal with respect to the reagent containing member flat surface, thereby measuring the depth of color of the reagent containing member.

17. The color measuring apparatus according to claim 16, wherein the color depth measuring unit measures the reflected intensity of light by receiving the same through optical fiber.