Portable device for total gossypol measurement

• US 20050128485A1
• Filed: 12/12/2003
• Published: 06/16/2005
• Est. Priority Date: 12/12/2003
• Status: Abandoned Application

• Current Assignee: Council of Scientific & Industrial Research
• Original Assignee: Council of Scientific & Industrial Research
• Inventors: Singh, Mewa, Mahapatra, Prasant Kumar, Singla, Madan Lal
• Application Number:
  US10/735,555
• Publication Number:
  US 20050128485A1
• Time in Patent Office: Days
• US Class Current: 356/432
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/59 Transmissivity G01N21/25 takes precedence
• CPC Class Codes: G01N 2201/062 LED's

Abstract

The measurement of gossypol in cotton seed products using conventional methods namely spectrophotmetric, NMR methods, chemiluminsecent method, gas-liquid chromatography polarography, thin layer chromatography and paper chromatography are tedious, time-consuming, high cost and needs calibrated systems. In the present invention, a low cost and portable system is devised and the concentration of total gossypol is directly displayed on LCD in mgl⁻¹ or ppm from the test solution and no standard solution is required to make calibration curve for measuring the absorbance.

Claims

1. A direct display portable device for measurement of gossypol concentration after colour development, said apparatus comprising:

a) a light source for emitting light of specific wavelength,

b) a means to control intensity of light from the light source,

c) a glass cuvettee for holding the test sample through which the light is passed,

d) a cuvette sample holder for holding the glass cuvettee,

e) a photo detector for detecting the light transmitted by test sample,

f) a means to convert the output current signal from the photo detector to voltage,

g) a log amplifier for amplifying the output signal of the converter,

h) a calibration circuit enabling display on a display unit, and

i) a power supply for supplying power to different components of the device.

2. The device as claimed in claim 1, wherein gossypol is selected from the group consisting of deoiled cake, cottonseed and cottonseed oil.

3. The device as claimed in claim 1, wherein the determination of the concentration of the gossypol concentration is in milligram/litre and parts per million (ppm) in the display unit of atleast 3½

digit display.

4. The device as claimed in claim 1, wherein the light source is an LED of 5 mm having intensity of 125 mcd, view angle of 24°

, peak wavelength of 635 nm and power dissipation of 50 mW.

5. The device as claimed in claim 1, wherein the means used to control the intensity of light from LED is a multiturn potentiometer to get 100% transmittance (0% absorbance) of light through distilled water in the cuvette.

6. The device as claimed in claim 1, wherein the cuvettee used is made up from Borosil glass.

7. The device as claimed in claim 1, wherein path length of the glass cuvettee is of 17 mm.

8. The device as claimed in claim 1, wherein the cuvettee used is screw capped having a diameter of 17 mm, height of 5 cm and capacity of 6 ml.

9. The device as claimed in claim 1, wherein cuvettee holder is made up from aluminium alloy, which is blackened.

10. The device as claimed in claim 1, wherein the photo detector BPW 21 having package of TO 5, effective area of detection of 5.9 mm diameter wavelength range of 460-750 nm and sensitivity of 7 nA/lux is used to detect the transmitted/absorbed light through the test solution.

11. The device as claimed in claim 1, wherein the current to voltage converter produces voltage which is directly proportional to current flowing through the converter, which inturn is proportional to the light intensity falling on the detector.

12. The device as claimed in claim 1, wherein the means to convert the current signal to voltage signal is a current/voltage converter.

13. The device as claimed in claim 1, wherein the log amplifier is TL 441 IC.

14. The device as claimed in claim 1, wherein the calibration circuit is a 10 K POT and after log amplification, voltage output is calibrated in terms of gossypol concentration in the test solution.

15. The device as claimed in claim 1, wherein the power supply is a 6-Volt battery for the log amplifier, photo detector and other integrated chips and a 9-Volt supply for the display unit.

16. The device as claimed in claim 1, wherein the results are displayed on the display unit.

17. The device as claimed in claim 1, wherein the results are displayed on computer through CC⁺ large.

18. The device as claimed in claim 1, wherein a software VISIDAQ is used for data acquisition, calculation and control application.

19. The device as claimed in claim 1, wherein the present device is based on Lambert Beer'"'"'s Law.

20. The device as claimed in claim 1 is low cost, portable, rugged and measures gossypol in the range of ±

2% error.