Liquid permeable composition in dry reagent devices
First Claim
1. A device for detection of an analyte in a liquid sample comprising a liquid permeable layer capable of adhesion for transferring portions of said liquid sample and disposed between absorbent or non-absorbent layers, at least one of said layers containing reagents for detecting said analyte, said layer comprising a blend of an aqueous polymer dispersion and a water soluble polymer which has been dried at a temperature of about 40°
- C. to 90°
C. to form said liquid permeable layer having a dry thickness of about 3 to 100 mils (75 to 2,540 μ
m), said liquid permeable layer being applied as a coating between absorbent or non-absorbent layers and the combined coating and absorbent or non-absorbent layers pressed together with a pressure of about 3 to 100 psi sufficient to cause said layers to adhere and then dried at temperatures such that the liquid permeable layer adheres to the adjacent said absorbent or non-absorbent layers while retaining liquid permeability.
3 Assignments
0 Petitions
Accused Products
Abstract
A device for detecting an analyte in a fluid sample includes a liquid permeable composition for making a physical separation between compositions of the sample or for reacting with components. The liquid permeable composition has adhesive properties and can be used to make a multi-layered test strip or the composition can be used in the sample wells of microfluidic devices.
24 Citations
50 Claims
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1. A device for detection of an analyte in a liquid sample comprising a liquid permeable layer capable of adhesion for transferring portions of said liquid sample and disposed between absorbent or non-absorbent layers, at least one of said layers containing reagents for detecting said analyte, said layer comprising a blend of an aqueous polymer dispersion and a water soluble polymer which has been dried at a temperature of about 40°
- C. to 90°
C. to form said liquid permeable layer having a dry thickness of about 3 to 100 mils (75 to 2,540 μ
m), said liquid permeable layer being applied as a coating between absorbent or non-absorbent layers and the combined coating and absorbent or non-absorbent layers pressed together with a pressure of about 3 to 100 psi sufficient to cause said layers to adhere and then dried at temperatures such that the liquid permeable layer adheres to the adjacent said absorbent or non-absorbent layers while retaining liquid permeability. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- C. to 90°
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14. A method of analyzing a liquid sample wherein said sample is contacted with dry reagents in layers containing said reagents which react with analytes in said sample to provide a detectable response characterized by including a liquid permeable layer comprising a blend of an aqueous polymer dispersion and a water soluble polymer for transferring portions of said liquid sample between said layers and adhering to said layers, said liquid permeable layer being applied as a liquid coating and dried at a temperature of about 40°
- C. to90°
C. to form said liquid permeable layer having a dry thickness of about 3 to 100 mils (75 to 2,540 μ
m), and then pressed with a pressure of about 3 to 100 psi sufficient to cause said layers to adhere and dried in place between said layers such that the liquid permeable layer adheres to said layers while retaining liquid permeability. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
- C. to90°
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28. A multi-layer device for detection of an analyte in a fluid sample comprising:
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(a) at least a first absorbent or non-absorbent layer for receiving said fluid sample;
(b) at least a second absorbent or non-absorbent layer for receiving a portion of said sample from said first layer; and
(c) a liquid permeable adhesive layer disposed between said at least first and second layers, said adhesive being diffusable to fluids and comprising a blend of an aqueous polymer dispersion and a water soluble polymer which has been cast as a liquid coating and dried at a temperature of about 40°
C. to 90°
C. to form said liquid permeable layer having a dry thickness of about 3 to 100 mils (75 to 2,540 μ
m), then pressed and dried in place between said first and second layers with a pressure of about 3 to 100 psi sufficient to cause said layers to adhere and at temperatures such that said adhesive layer adheres to said first and second layers while retaining liquid permeability, at least one of said combined layers of (a), (b), (c) containing reagents and being capable of detecting said analyte. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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Specification