Sequencing on perforated membranes
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Abstract
The invention concerns a method and device for sequencing nucleic acid molecules on a perforated membrane. The invention can be used in particular for multiplex sequencing.
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Citations
51 Claims
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1-26. -26. (canceled)
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27. A method for sequencing nucleic acids comprising the steps:
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(a) providing a nucleic acid molecule to be sequenced which contains a plurality of flourescence-labelling groups, (b) proving a membrane structure through which at least one channel extends having a suitable diameter for the passage of single nucleic acid molecules, wherein an enzyme which catalyses the cleavage of single nucleotide building blocks from a nucleic acid molecule is immobilized on the membrane structure, (c) passing the nucleic acid molecule to be sequenced into and/or through the channel under conditions that allow successive cleavage of single nucleotide building blocks from the nucleic acid molecule and (d) determining the base sequence of the nucleic acid molecule by fluorescence measurement. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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48. A carrier for sequencing nucleic acids, comprising:
- a membrane structure through which a channel extends which has a diameter suitable for the passage of single nucleic acid molecules, an enzyme which catalyses cleavage of single nucleotide building blocks from a nucleic acid molecule being immobilized on the membrane structure.
- View Dependent Claims (49, 50, 51)
Specification