Charge tags and the separation of nucleic acid molecules
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Abstract
The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
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Citations
85 Claims
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1-50. -50. (canceled)
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51. A method of separating nucleic acid molecules, comprising the steps of:
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a) treating a charge-balanced oligonucleotide containing a charge tag under conditions such that a charge-unbalanced oligonucleotide containing said charge tag is produced, wherein said charge-unbalanced oligonucleotide is contained in a reaction mixture; and
b) separating said charge-unbalanced oligonucleotide from said reaction mixture. - View Dependent Claims (52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71)
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72. A method of separating nucleic acid molecules, comprising the steps of:
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a) treating a plurality of charge-balanced oligonucleotides, each containing different charge tags, under conditions such that two or more charge-unbalanced oligonucleotides containing said charge tags are produced, wherein said charge-unbalanced oligonucleotides are contained in a reaction mixture; and
b) separating said charge-unbalanced oligonucleotides from said reaction mixture. - View Dependent Claims (73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85)
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Specification