Method of identifying a gene product
First Claim
1. A method of identifying a gene product, wherein the method comprises:
- (a) providing a multiplicity of cells comprising a first gene product;
(b) introducing into the multiplicity of cells a library of second nucleic acid sequences encoding second gene products;
(c) expressing the second nucleic acid sequences to produce the second gene products such that the first gene product and at least one of the second gene products contact;
(d) causing at least one complex to form between the first gene product, an affinity molecule, and at least one of the second gene products;
(e) retrieving the complex; and
(f) identifying at least one second gene product of the complex.
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Abstract
The present invention provides a method of identifying a gene product. The method comprises providing a multiplicity of cells comprising a first gene product. Preferably, the first gene product is produced in the multiplicity of cells by expressing a first exogenous nucleic acid sequence encoding the first gene product. A library of second nucleic acid sequences encoding second gene products is then introduced into the multiplicity of cells. The second nucleic acid sequences are expressed in the multiplicity of cells to produce the second gene products such that the first gene product and at least one of the second gene products contact. The method further comprises causing a complex to form between the first gene product, an affinity molecule that binds the first gene product, and at least one of the second gene products, and subsequently retrieving the complex. At least one second gene product of the complex then is identified.
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Citations
24 Claims
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1. A method of identifying a gene product, wherein the method comprises:
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(a) providing a multiplicity of cells comprising a first gene product;
(b) introducing into the multiplicity of cells a library of second nucleic acid sequences encoding second gene products;
(c) expressing the second nucleic acid sequences to produce the second gene products such that the first gene product and at least one of the second gene products contact;
(d) causing at least one complex to form between the first gene product, an affinity molecule, and at least one of the second gene products;
(e) retrieving the complex; and
(f) identifying at least one second gene product of the complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method of identifying a gene product, wherein the method comprises:
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(a) providing a viral vector comprising a first nucleic acid sequence encoding a first gene product;
(b) providing a library of viral vectors, wherein each viral vector of the library comprises a second nucleic acid sequence encoding a second gene product;
(c) transducing a multiplicity of host cells with the viral vector comprising the first nucleic acid sequence and the library of viral vectors comprising second nucleic acid sequences, wherein the host cells are permissive for expression of the first nucleic acid sequence and the second nucleic acid sequences and production of the first gene product and the second gene products;
(d) expressing the first nucleic acid sequence and the second nucleic acid sequences such that the first gene product and at least one of the second gene products contact;
(e) causing at least one complex to form between the first gene product, an affinity molecule, and at least one of the second gene products;
(f) retrieving the formed complex; and
(g) identifying at least one second gene product of the complex. - View Dependent Claims (20, 21, 22, 23, 24)
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Specification