Fully integrated protein lab-on-a-chip with smart microfluidics for spot array generation
First Claim
1. A method to fabricate a protein micro-array used in a biochip, said method comprising selectively modifying a surface of a plastic substrate to generate a modified surface having a plurality of hydrophobic spots on a hydrophilic background.
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Abstract
Techniques for the fabrication of fully-integrated lab-on-a-chips (or biochips) specifically oriented towards point-of-care detection of biomolecules using immunoassay based detection techniques are disclosed. A primary task for the development of such biochips is the development of techniques to precisely deposit and localize the capture antibody on pre-determined locations over the biochip. The use of selective surface modification, specifically control over the surface energy, to achieve localized adsorption of the capture antibody is disclosed. Another approach, also disclosed, describes the use of smart passive microfluidics to confine the flow of the capture antibody along certain paths of the biochip and thereby control the locations over which the capture antibody is adsorbed. Furthermore, the use of an integrated microlens array as means of enhancing the detection sensitivity of the biochip is also disclosed.
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Citations
34 Claims
- 1. A method to fabricate a protein micro-array used in a biochip, said method comprising selectively modifying a surface of a plastic substrate to generate a modified surface having a plurality of hydrophobic spots on a hydrophilic background.
- 10. A protein micro-array used in a biochip, said micro-array comprising a plastic substrate having a selectively modified surface wherein said selectively modified surface includes a plurality of hydrophobic spots arranged on a hydrophilic background.
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15. An integrated protein biochip, said biochip comprising:
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a protein micro-array; and
a micro-fluidic system to transport fluids to said protein micro-array. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method to fabricate an integrated protein biochip, said method comprising:
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fabricating a protein micro-array having a plurality of hydrophobic spots on a hydrophilic background; and
fabricating a micro-fluidic system connecting to said protein micro-array to deliver fluids to said plurality of hydrophobic spots of said protein micro-array. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34)
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Specification