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Isolation of bone marrow fraction rich in connective tissue growth components and the use thereof to promote connective tissue formation

  • US 20050130301A1
  • Filed: 07/08/2004
  • Published: 06/16/2005
  • Est. Priority Date: 07/09/2003
  • Status: Abandoned Application
First Claim
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1. A method for obtaining a bone marrow fraction, comprising:

  • a) centrifuging a biological sample including bone marrow and whole blood to provide a separation of components of the sample based on density, said separation providing the following fractions in decreasing order of density;

    (i) a fraction rich in blood cells;

    (ii) a buffy coat fraction;

    (iii) a platelet rich fraction; and

    (iv) a platelet poor fraction; and

    b) isolating the buffy coat fraction alone or in combination with all or part of the platelet rich fraction to form an isolate rich in connective tissue growth promoting components.

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