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Ultra high throughput microfluidic analytical systems and methods

  • US 20050135655A1
  • Filed: 01/13/2005
  • Published: 06/23/2005
  • Est. Priority Date: 03/27/2000
  • Status: Abandoned Application
First Claim
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1. An illumination and detection system for use in illuminating a plurality of samples in a plurality of microchannels located in a detection region on a microfluidic device, and for detecting radiation emitted from the detection region, wherein the microchannels are substantially parallel along a first direction within the detection region, the system comprising:

  • an illumination source for providing an excitation beam having two or more excitation wavelengths;

    focussing optics for focussing the excitation beam onto a first plane defined by the plurality of microchannels in the detection region such that the focussed excitation beam is elongated, having a major axis substantially perpendicular to the first direction, wherein the excitation beam impinges upon the detection region at a non-normal angle of incidence relative to the first plane, and wherein the excitation beam simultaneously excites the samples in at least two of the microchannels so as to cause the excited samples to emit radiation;

    two or more detectors, wherein each detector detects a specific range of radiation wavelengths; and

    detection optics for directing radiation from the samples toward the detectors such that the wavelengths of the emitted radiation within each specific radiation wavelength range are directed toward the corresponding detector.

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