Primer extension detection methods on active electronic microarrays
First Claim
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1. A method of detecting the extent of hybridization of a nucleic acid in a sample to a probe nucleic acid sequence, the method comprising:
- (a) electronically hybridizing the nucleic acid in a sample to a nucleic acid probe bound to a support at a predetermined location;
(b) utilizing the hybridized nucleic acid as a template in a nucleic acid polymerase reaction to extend the bound probe, whereby a labeled nucleotide is incorporated into the extended probe; and
(c) detecting the labeled nucleotide incorporated into the extended bound probe at the predetermined location.
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Abstract
This invention relates to methods for detecting the extent of hybridization of a nucleic acid in a sample to a probe nucleic acid sequence by electronically hybridizing the nucleic acid in the sample to the probe, utilizing the hybridized nucleic acid as a template in a nucleic acid polymerase reaction to extend the bound probe and incorporate a labeled nucleotide, and detecting the labeled product. This invention also relates to methods of detecting the extent of hybridization of a plurality of nucleic acids in a sample to a plurality of nucleic acid probes using similar methods.
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Citations
21 Claims
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1. A method of detecting the extent of hybridization of a nucleic acid in a sample to a probe nucleic acid sequence, the method comprising:
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(a) electronically hybridizing the nucleic acid in a sample to a nucleic acid probe bound to a support at a predetermined location;
(b) utilizing the hybridized nucleic acid as a template in a nucleic acid polymerase reaction to extend the bound probe, whereby a labeled nucleotide is incorporated into the extended probe; and
(c) detecting the labeled nucleotide incorporated into the extended bound probe at the predetermined location. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of detecting the extent of hybridization of a plurality of nucleic acids in a sample to a plurality of nucleic acid probes, the method comprising:
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(a) electronically hybridizing the nucleic acid in the sample to a plurality of nucleic acid probes bound to a support at a two or more predetermined locations, wherein;
i) the nucleic acids in the sample are electronically hybridized to nucleic acid probes at two or more locations on the support, and ii) the sequence of at least one nucleic acid probe on a first location is different from the sequence of the nucleic acid probes on a second location. (b) utilizing the hybridized nucleic acids as a templates in a nucleic acid polymerase reaction to extend the bound probes, whereby a labeled nucleotide is incorporated into the extended probes; and
(c) detecting the labeled nucleotide incorporated into the extended bound probes at the predetermined location. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification