Screening assays
First Claim
Patent Images
1. A method of screening for compounds that reduce and/or prevent obesity comprising a) contacting a cell expressing a gene selected from the group consisting of Seq. ID Nos. 1-6 with a compound;
- b) measuring the expression of said gene, or the expression of a polypeptide encoded by said gene; and
c) determining whether the compound up-regulates the expression of said gene or of the polypeptide encoded by said gene.
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Abstract
The present invention relates to novel targets for identifying compounds that may be useful for the prevention and treatment of obesity.
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Citations
35 Claims
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1. A method of screening for compounds that reduce and/or prevent obesity comprising
a) contacting a cell expressing a gene selected from the group consisting of Seq. ID Nos. 1-6 with a compound; -
b) measuring the expression of said gene, or the expression of a polypeptide encoded by said gene; and
c) determining whether the compound up-regulates the expression of said gene or of the polypeptide encoded by said gene. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of screening for compounds that reduce and/or prevent obesity comprising
a) contacting a cell expressing a gene selected from the group consisting of Seq ID No. 25-37 with a compound; -
b) measuring the expression of said gene, or the expression of a polypeptide encoded by said gene; and
c) determining whether the compound up-regulates the expression of said gene or of the polypeptide encoded by said gene. - View Dependent Claims (10, 11, 12, 13, 14, 15)
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9. A method of screening for compounds that reduce and/or prevent obesity comprising
a) contacting a cell expressing a gene selected from the group consisting of Seq. ID Nos. 7-12 with a compound; -
b) measuring the expression of said gene, or the expression of a polypeptide encoded by said gene; and
c) determining whether the compound down-regulates the expression of said gene or of a polypeptide encoded by said gene.
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16. A method of screening for compounds that reduce and/or prevent obesity comprising
a) contacting a cell expressing a gene selected from the group consisting of Seq ID No. 38 to 85 with a compound; -
b) measuring the expression of said gene, or the expression of a polypeptide encoded by said gene; and
c) determining whether the compound down-regulates the expression of said gene or of a polypeptide encoded by said gene.
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17. A method of screening for compounds that reduce and/or prevent obesity comprising:
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 13 to 18 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by agonizing said polypeptide is a compound which causes an increase in activity of said polypeptide.
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 13 to 18 with a compound; and
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18. A method of screening for compounds that reduce and/or prevent obesity comprising:
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 86 to 98 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by agonizing said polypeptide is a compound which causes an increase in activity of said polypeptide.
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 86 to 98 with a compound; and
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19. A method of screening for compounds that reduce and/or prevent obesity comprising:
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 19 to 24 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by antagonizing said polypeptide is a compound which causes a decrease in activity of said polypeptide.
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 19 to 24 with a compound; and
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20. A method of screening for compounds that reduce and/or prevent obesity comprising:
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 99 to 146 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by antagonizing said polypeptide is a compound which causes a decrease in activity of said polypeptide.
- a) contacting a polypeptide selected from the group consisting of Seq ID No. 99 to 146 with a compound; and
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21. A method for screening of compounds that reduce and/or prevent obesity comprising:
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No. 13 to 18 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by agonizing said polypeptide is a compound which causes an increase in activity of said polypeptide.
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No. 13 to 18 with a compound; and
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22. A method for screening of compounds that reduce and/or prevent obesity comprising:
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No.86 to 98 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by agonizing said polypeptide is a compound which causes an increase in activity of said polypeptide.
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No.86 to 98 with a compound; and
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23. A method for screening of compounds that reduce and/or prevent obesity comprising:
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No. 19 to 24 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by antagonizing said polypeptide is a compound which causes a decrease in activity of said polypeptide.
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No. 19 to 24 with a compound; and
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24. A method for screening of compounds that reduce and/or prevent obesity comprising:
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No. 99 to 146 with a compound; and
b) determining the activity of said polypeptide;
wherein a compound which reduces and/or prevents obesity by antagonizing said polypeptide is a compound which causes a decrease in activity of said polypeptide.
- a) contacting a cell expressing a nucleic acid encoding a polypeptide selected from the group consisting of Seq ID No. 99 to 146 with a compound; and
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25. A method of screening for compounds that bind to a polypeptide selected from the group consisting of the polypeptides of Seq ID No. 13 to 24, comprising the steps of
a) contacting a compound with said polypeptide; - and
b) determining the ability of said compound to bind to said polypeptide.
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26. A method of screening for compounds that bind to a polypeptide selected from the group consisting of the polypeptides of Seq ID No.86 to 146, comprising the steps of
a) contacting a compound with said polypeptide; - and
b) determining the ability of said compound to bind to said polypeptide.
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34. A pharmaceutical composition for the modulation of body weight, comprising a compound that modulates the expression or activity of a polypeptide selected from the group consisting of Seq ID No. 13 to 24, mixed with a pharmaceutically acceptable carrier.
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35. A pharmaceutical composition for the modulation of body weight, comprising a compound that modulates the expression or activity of a polypeptide selected from the group consisting of Seq ID No. 86 to 146, mixed with a pharmaceutically acceptable carrier.
Specification