Flow-through assay devices
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Abstract
A flow-through assay device capable of detecting the presence or quantity of an analyte of interest is provided that is accurate, reliable, and easy-to-use. The device contains a substrate printed with a channel to facilitate the flow of a test sample to a detection working electrode. The detection working electrode communicates with affinity reagents, such as redox mediators and capture ligands. For instance, capture ligands that are specific binding members for the analyte of interest are applied to the detection electrode to serve as the primary location for detection of the analyte.
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Citations
91 Claims
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1-49. -49. (canceled)
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50. A method for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:
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i) forming a flow-through assay device by a method comprising;
a) printing a fluidic channel onto a surface of a substrate, wherein said channel has opposing walls that are raised above the surface of the substrate;
b) forming a detection working electrode on said surface of said substrate, said detection working electrode being in fluid communication with said fluidic channel; and
c) treating a surface of said detection working electrode with a specific binding capture ligand for the analyte; and
ii) contacting the test sample with said fluidic channel of said assay device. - View Dependent Claims (51, 52)
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53. A method of forming a flow-through assay device for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:
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i) printing a fluidic channel onto a surface of a substrate, wherein said channel has opposing walls that are raised above the surface of the substrate;
ii) forming a detection working electrode on said surface of said substrate, said detection working electrode being in fluid communication with said fluidic channel; and
iii) treating a surface of said detection working electrode with a specific binding capture ligand for the analyte. - View Dependent Claims (54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75)
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76. A method of forming a flow-through assay device for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:
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i) printing a fluidic microchannel onto a surface of a substrate, said fluidic microchannel being formed from a dielectric material and having opposing walls that are raised above the surface of the substrate, wherein said microchannel has a characteristic dimension of from about 0.5 to about 500 micrometers;
ii) forming a detection working electrode on said surface of said substrate, said detection working electrode being in fluid communication with said fluidic microchannel; and
iii) treating a surface of said detection working electrode with a specific binding capture ligand for the analyte. - View Dependent Claims (77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91)
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Specification