Embryonic epithelial cells

• US 20050136536A1
• Filed: 09/15/2004
• Published: 06/23/2005
• Est. Priority Date: 09/15/2003
• Status: Abandoned Application

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Anderson, Daniel G., Langer, Robert S., Levenberg, Shulamit
• Application Number:
  US10/941,390
• Publication Number:
  US 20050136536A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C12N 2501/385 of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 2537/00 Supports and/or coatings for cell culture characterised by physical or chemical treatment
• CPC Class Codes: C12N 5/063 Kereatinocyte stem cells; Keratinocyte progenitors

Abstract

A population of embryonic epithelial cells produced in vitro from embryonic stem cells. In one embodiment, at least 45% of the cells express cytokeratin, for example, cytokeratin-7.

Claims

1. A population of embryonic epithelial cells produced in vitro from embryonic stem cells.

2. The population of claim 1, wherein at least 45% of the cells express cytokeratin.

3. The population of claim 2, wherein at least 55% of the cells express cytokeratin.

4. The population of claim 3, wherein at least 65% of the cells express cytokeratin.

5. The population of claim 4, wherein at least 75% of the cells express cytokeratin.

6. The population of claim 5, wherein at least 85% of the cells express cytokeratin.

7. The population of claim 6, wherein at least 95% of the cells express cytokeratin.

8. The population of claim 1, wherein at least 45% of the cells express cytokeratin 7.

9. A population of cytokeratin-positive cells produced in vitro from embryonic stem cells.

10. The population of claim 9, wherein at least 45% of the cells express cytokeratin.

11. The population of claim 10, wherein at least 55% of the cells express cytokeratin.

12. The population of claim 11, wherein at least 65% of the cells express cytokeratin.

13. The population of claim 12, wherein at least 75% of the cells express cytokeratin.

14. The population of claim 13, wherein at least 85% of the cells express cytokeratin.

15. The population of claim 14, wherein at least 95% of the cells express cytokeratin.

16. The population of claim 9, wherein at least 45% of the cells express cytokeratin 7.

17. A population of cytokeratin-7 positive cells, produced by the step of:

exposing a population of embryonic stem cells to retinoic acid.

18. The population of claim 17, wherein the population is exposed to retinoic acid in the presence of serum.

19. The population of claim 17, wherein the population of embryonic stem cells is seeded on a cell support substrate.

20. The population of claim 18, wherein the cell support substrate comprises polymerized 1,4 butandiol dimethacrylate, diethylene glycol dimethacrylate, phenylene diacrylate 1,3

21. The population of claim 18, wherein the cell support substrate comprises a polymer of one or more monomers having a structure selected from

22. A composition, comprising:

a cell support substrate; and

human embryonic epithelial cells supported by the cell support substrate.

23. The composition of claim 22, wherein the cell support substrate comprises polymerized 1,4 butanediol dimethacrylate, diethylene glycol dimethacrylate, phenylene diacrylate 1,3,

24. The composition of claim 22, wherein the cell support substrate comprises a polymer of one or more monomers having a structure selected from

25. The composition of claim 22, further comprising retinoic acid.

26. The composition of claim 22, further comprising serum.

27. The composition of claim 22, wherein at least 45% of the cells express cytokeratin.

28. The composition of claim 27, wherein at least 55% of the cells express cytokeratin.

29. The composition of claim 28, wherein at least 65% of the cells express cytokeratin.

30. The composition of claim 29, wherein at least 75% of the cells express cytokeratin.

31. The composition of claim 30, wherein at least 85% of the cells express cytokeratin.

32. The composition of claim 31, wherein at least 95% of the cells express cytokeratin.

33. The composition of claim 22, wherein at least 45% of the cells express cytokeratin 7.

34. A method of enriching a population of embryonic stem cells with epithelial-like cells, comprising:

providing a population of human embryonic stem cells; and

culturing the stem cells on an acrylate polymer in a culture medium including retinoic acid.

35. The method of claim 34, wherein the culture medium includes serum.

36. The method of claim 34, wherein the acrylate polymer is formed from one or more monomers having a structure selected from

37. The method of claim 34, wherein the acrylate polymer is selected from polymerized 1,4 butanediol dimethacrylate, diethylene glycol dimethacrylate, phenylene diacrylate 1,3,

38. The method of claim 34, wherein providing comprises:

culturing embryonic stem cells under conditions where embryoid bodies are formed; and

dissociating the embryoid bodies.

39. The method of claim 34, wherein at least 45% of the cells express cytokeratin.

40. The method of claim 39, wherein at least 55% of the cells express cytokeratin.

41. The method of claim 40, wherein at least 65% of the cells express cytokeratin.

42. The method of claim 41, wherein at least 75% of the cells express cytokeratin.

43. The method of claim 42, wherein at least 85% of the cells express cytokeratin.

44. The method of claim 43, wherein at least 95% of the cells express cytokeratin.

45. The method of claim 34, wherein at least 45% of the cells express cytokeratin 7.