Diagnostic devices and apparatus for the controlled movement of reagents without membranes
First Claim
1. A device for detecting one or more analytes of interest, comprising:
- a) a sample addition zone on a surface of said device;
b) an array of posts protruding from between 10 nm and 0.5 mm on said device surface; and
c) one or more capture zones on said device surface, said capture zones comprising reagents to bind to or react with said one or more analytes of interest;
wherein said array of posts and one or more capture zones are in fluid communication with said sample addition zone, and wherein said posts are configured and arranged to provide flow of a sample fluid applied to said sample addition zone through said array of posts by capillary force.
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Accused Products
Abstract
The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.
158 Citations
13 Claims
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1. A device for detecting one or more analytes of interest, comprising:
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a) a sample addition zone on a surface of said device;
b) an array of posts protruding from between 10 nm and 0.5 mm on said device surface; and
c) one or more capture zones on said device surface, said capture zones comprising reagents to bind to or react with said one or more analytes of interest;
wherein said array of posts and one or more capture zones are in fluid communication with said sample addition zone, and wherein said posts are configured and arranged to provide flow of a sample fluid applied to said sample addition zone through said array of posts by capillary force. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification