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Methods for nucleic acid isolation and kits using solid phase material

  • US 20050142571A1
  • Filed: 05/24/2004
  • Published: 06/30/2005
  • Est. Priority Date: 12/24/2003
  • Status: Abandoned Application
First Claim
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1. A method of isolating nucleic acid from a sample, the method comprising:

  • providing a sample comprising nucleic acid-containing material and inhibitors;

    forming a concentrated region of the sample;

    wherein the concentrated region of the sample comprises nucleic acid-containing material and inhibitors;

    substantially separating the concentrated region from a less concentrated region of the sample;

    contacting the separated concentrated region of the sample with a solid phase material to preferentially adhere at least a portion of the inhibitors to the solid phase material, wherein the solid phase material comprises a polytetrafluoroethylene fibril matrix, sorptive particles enmeshed in the matrix, and a coating reagent coated on the solid phase material;

    wherein the coating reagent is selected from the group consisting of a surfactant, a strong base, a polyelectrolyte, a selectively permeable polymeric barrier, and combinations thereof;

    optionally lysing the nucleic acid-containing material to release nucleic acid before, simultaneous with, or after contacting the separated concentrated region of the sample with the solid phase material; and

    separating at least a portion of the nucleic acid-containing material and/or nucleic acid from the solid phase material having at least a portion of the inhibitors adhered thereto.

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