Colloid compositions for solid phase biomolecular analytical, preparative and identification systems
First Claim
1. A liquid composition comprising a colloidal suspension of a biomolecule-binding matrix material dispersed in a liquid, wherein particles of said matrix material in said colloidal suspension are of a defined particle size;
- and replicate copies of a biomolecule, wherein said biomolecules are distributed throughout said colloidal suspension and are bound to said matrix material particles.
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Accused Products
Abstract
A liquid composition comprising a colloidal suspension of a biomolecule-binding matrix material (preferably nitrocellulose) dispersed in a liquid, with particles of the matrix material being of a defined particle size, and replicate copies of a biomolecule, e.g., protein or nucleic acid probes, which are distributed, preferably uniformly, throughout the colloidal suspension and are bound to the matrix material particles, is disclosed. The liquid composition of the invention can be used directly for sample analysis or preparation of biomolecules, or aliquots of the composition can be spotted onto a support to form a microporous matrix system or microarray for analysis or preparation of biomolecules. Compositions and microarrays according to the invention are useful in any type of analytical or preparative procedure relating to biomolecules. They are particularly useful, e.g., in methods for detecting a biomolecule analyte in a liquid sample, methods for determining the presence of a particular nucleic acid sequence within a liquid sample and methods for determining the presence of a drug candidate molecule in a liquid sample. The invention further comprises kits for practicing the various methods of the invention.
10 Citations
49 Claims
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1. A liquid composition comprising
a colloidal suspension of a biomolecule-binding matrix material dispersed in a liquid, wherein particles of said matrix material in said colloidal suspension are of a defined particle size; - and
replicate copies of a biomolecule, wherein said biomolecules are distributed throughout said colloidal suspension and are bound to said matrix material particles. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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16. A liquid composition comprising
a colloidal suspension of a biomolecule-binding matrix material dispersed in a liquid, wherein particles of said matrix material in said colloidal suspension are of a defined particle size.
- 33. A microarray having a multiplicity of spots, wherein the composition of each of said spots comprises a biomolecule-binding matrix material, a first biomolecule bound to said matrix material and a second biomolecule bound to said matrix material, wherein the concentration of said matrix material and the concentration of said first biomolecule varies among the said spots.
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38. A microarray having a multiplicity of spots, said microarray characterized in that the composition of each spot comprises:
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a biomolecule-binding matrix material;
a first biomolecule bound to said matrix material; and
a second biomolecule bound to said matrix material, wherein said relative concentrations of said matrix material and said first biomolecule may vary among said multiplicity of said spots and wherein the quantity of said biomolecule-binding matrix material present in each spot is determined independently from spot to spot throughout said multiplicity of spots.
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39. A microarray on a solid support surface, said microarray having a multiplicity of spots, said microarray characterized in that the composition of each spot comprises:
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a biomolecule-binding matrix material; and
a first biomolecule bound to said matrix material, wherein the thickness of each of said spots is less than 10 μ
m. - View Dependent Claims (40)
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41. A microarray on a solid support surface, said microarray having a multiplicity of spots, said microarray characterized in that:
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the composition of each of said multiplicity of spots comprises a biomolecule-binding matrix material and a biomolecule bound to said matrix material;
each of said multiplicity of spots is greater than or equal to 150 μ
m in diameter; and
the volume of each of said multiplicity of spots is less than 100 nl. - View Dependent Claims (42)
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Specification