Method of measuring blood component and sensor used in the method
First Claim
1. A method of measuring a blood component, comprising:
- causing a redox reaction between the blood component and an oxidoreductase in the presence of a mediator;
detecting a redox current generated by the redox reaction by electrodes; and
converting the detected current value into an amount of the blood component, wherein when detecting the redox current, an erythrocyte is hemolyzed so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit.
7 Assignments
0 Petitions
Accused Products
Abstract
A sensor for blood component analysis that can correct the effect of a hematocrit easily is provided. The sensor includes an analysis portion including a working electrode, a counter electrode, and a reagent portion. The reagent portion includes an oxidoreductase that reacts with the blood component and a mediator, and the blood component is measured by causing a redox reaction between the blood component and the oxidoreductase in the presence of the mediator and detecting a redox current generated by the redox reaction by the working electrode and the counter electrode. In this sensor, the reagent portion further includes a hemolyzing agent (e.g., sodium cholate) for hemolyzing an erythrocyte, and when detecting the redox current, the erythrocyte is hemolyzed with the hemolyzing agent so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit.
-
Citations
20 Claims
-
1. A method of measuring a blood component, comprising:
-
causing a redox reaction between the blood component and an oxidoreductase in the presence of a mediator;
detecting a redox current generated by the redox reaction by electrodes; and
converting the detected current value into an amount of the blood component, wherein when detecting the redox current, an erythrocyte is hemolyzed so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A sensor for measuring a blood component, comprising an analysis portion, the analysis portion comprising:
-
a working electrode;
a counter electrode; and
a reagent portion, the reagent portion comprising an oxidoreductase that reacts with the blood component and a mediator, the blood component being measured by causing a redox reaction between the blood component and the oxidoreductase in the presence of the mediator and detecting a redox current generated by the redox reaction by the working electrode and the counter electrode, wherein the reagent portion further comprises a hemolyzing agent for hemolyzing an erythrocyte, and when detecting the redox current, the erythrocyte is hemolyzed with the hemolyzing agent so as to cause hemoglobin released to an outside of the erythrocyte to react with the mediator and a current generated by this reaction also is detected to correct an effect of a hematocrit. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification