Phantom calibration device for low level light imaging systems

• US 20050145786A1
• Filed: 11/24/2004
• Published: 07/07/2005
• Est. Priority Date: 02/06/2002
• Status: Active Grant

• Current Assignee: Xenogen Corporation (Revvity, Inc.)
• Original Assignee: Xenogen Corporation (Revvity, Inc.)
• Inventors: Rice, Bradley W., Nilson, David G., Troy, Tamara L., Nantel, Normand P.
• Granted Patent: US 7,629,573 B2
• Time in Patent Office: Days
• US Class Current: 250/252.100
• CPC Class Codes: A61B 2560/0233 Optical standards
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0073 by tomography, i.e. reconstruction of 3D images from 2D projections A61B5/0066 takes precedence
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 21/278 Constitution of standards
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/763 Bioluminescence

Abstract

The present invention relates to a phantom device that simplifies usage and testing of a low intensity light imaging system. The phantom device includes a body and a light source internal to the body. The body comprises an optically selective material designed to at least partially resemble the optical behavior of mammalian tissue. Imaging the light source or phantom device may incorporate known properties of the optically selective material. Testing methods described herein assess the performance of a low-level light imaging system (such as the software) by processing light output by the phantom device and comparing the output against known results. The assessment builds a digital representation of the light source or test device and compares one or more components of the digital representation against one or more known properties for the light source or the test device.

Claims

1. A phantom device for use with a low-level light imaging system, the phantom device comprising:

a body including one or more surfaces and an optically selective material designed to at least partially resemble the optical behavior of mammalian tissue; and

a low intensity light source disposed within the body and configured to emit light from within the body, through the optically selective material and to the one or more surfaces,

wherein the light source is configured to emit a photon flux in the range of about 10⁴ to about 10¹³ photons/second.

2. The device of claim 1 wherein the optically selective material is designed or configured to resemble an optical absorption property of mammalian tissue.

3. The device of claim 1 wherein the optically selective material is designed or configured to resemble an optical scattering property of mammalian tissue.

4. The device of claim 1 wherein the optically selective material is substantially homogeneous.

5. The device of claim 1 wherein the low intensity light source comprises a radiation source configured to produce electrons that strike a light-emitting surface.

6. The device of claim 5 wherein the low intensity radiation light source is embedded in an end of a rod that comprises the optically selective material.

7. The device of claim 1 wherein the low intensity light source comprises an optical fiber having a first end configured to receive light from a light supply and a second end within the body.

8. The device of claim 7 further comprising a hole that receives a rod that includes a channel configured to receive the optical fiber and extending from a first end of the rod disposed proximate to the light supply to a second end of the rod within the body.

9. The device of claim 8 wherein the rod comprises the optically selective material.

10. The device of claim 7 wherein the light source comprises a light emitting diode that is configured to illuminate the first end of the optical fiber.

11. The device of claim 10 further comprising a second light emitting diode that is configured to illuminate the first end of the optical fiber, wherein the first and second light emitting diodes resemble a light spectrum for a biological reporter.

12. The device of claim 10 wherein the light emitting diode receives current from a current regulating source.

13. The device of claim 1 wherein a height for the body is designed to resemble a height of a mammal to be imaged.

14. The device of claim 13 wherein surface topography of the body is designed to match the surface topography of the mammal.

15. The device of claim 13 wherein the surface shape resembles a trapezoid.

16. The device of claim 13 wherein the light source emits light in the range of about 400 nanometers to about 1300 nanometers.

17. The device of claim 1 wherein the low intensity light source emits a photon flux in the range of about 10⁴ to about 10¹⁰ photons/second.

18. The device of claim 1 wherein the low intensity light source emits light from about 10⁻

15 to 10<sup>−

6</sup> watts of light.

19. The device of claim 1 wherein light radiance from the surface of the phantom device is from about 10³ to about 10¹¹ photons/second/centimeter squared/steradian.

20. The device of claim 1 wherein the low intensity light source emits a light spectrum that is substantially similar to a biological reporter.

21. The device of claim 20 wherein the optically selective material comprises titanium dioxide.

22. The device of claim 1 wherein the body is heterogeneous and includes at least two different materials.

23. A phantom device for testing a low-level light imaging system, the device comprising:

a body including one or more surfaces and a substantially homogeneous and optically selective material design ed to at least partially resemble the optical behavior of mammalian tissue, wherein the body includes a shape that resembles a mammal to be imaged and wherein a height for the body is designed to resemble a height of the mammal; and

a low intensity light source disposed within the body and configured to emit light from within the body, through the optically selective material and to the one or more surfaces.

24. The device of claim 23 wherein the optically selective material is configured to resemble an optical absorption property of mammalian tissue.

25. The device of claim 24 wherein the optically selective material comprises a reddish dye.

26. The device of claim 23 wherein the optically selective material is configured to resemble an optical scattering property of mammalian tissue.

27. The device of claim 26 wherein the optically selective material comprises a scattering particle.

28. The device of claim 25 wherein the low intensity light source emits light between about 1 nanowatt and about 10 nanowatts of light.

29. A method for testing a low-level light imaging system, the method comprising:

capturing an image of at least a portion of a phantom device disposed in a field of view for a camera included in the low-level light imaging system, the phantom device comprising a body including an optically selective material designed to at least partially resemble the optical behavior of mammalian tissue;

building a digital representation of a light source internal to the phantom device using data included in the image; and

comparing a component of the digital representation to a known property for the light source or the phantom device.

30. The method of claim 29 wherein the known property for the light source comprises one of:

intensity for the light source, a size of the light source, and a spectral pattern for the light source.

31. The method of claim 29 wherein the known property for the phantom device comprises one of:

a surface size for a surface of the phantom device, a location of the light source within the phantom device, and a spectral response for the optically selective material.

32. The method of claim 29 wherein the component comprises one of:

a flux from a surface of the phantom device, an estimated intensity of the light source, an estimated location of the light source within the phantom device, and an estimated size of the light source.

33. The method of claim 29 wherein the light source is reconstructed as a point.

34. The method of claim 29 wherein the light source is reconstructed as a complex source characterized spatially in three dimensions.

35. The method of claim 29 wherein reconstructing the digital representation of the light source employs one of:

an optical scattering representation for the optically selective material and an optical absorption representation for the optically selective material.

36. The method of claim 29 wherein building a digital representation comprises a diffuse tomography assessment for the light source that is stored in software run with the imaging system.

37. The method of claim 29 further comprising capturing a second image of the phantom device at a different wavelength.

38. The method of claim 29 further comprising:

altering a parameter used in reconstructing the digital representation; and

reconstructing a second digital representation using the altered parameter and the data included in the image.

39. The method of claim 29 further comprising assessing performance of the low-level light imaging system using the digital representation of the light source.

40. The method of claim 29 wherein the image comprises one of a photographic or a luminescent image.

41. The method of claim 29 further comprising calibrating the low-level light imaging system against a known intensity light source.

42. The method of claim 29 Wherein the light source emits fluorescent light.

43. A method for testing a low-level light imaging system, the method comprising:

calibrating the low-level light imaging system against a known intensity light source;

capturing an image of at least a portion of a phantom device disposed in a field of view for a camera included in the low-level light imaging system;

building a digital representation of a light source internal to the phantom device using data included in the image; and

comparing a component of the digital representation to a known property for the light source or the phantom device.

44. A computer readable medium including instructions for testing a low-level light imaging system, the instructions comprising:

instructions for capturing an image of at least a portion of a phantom device disposed in a field of view for a camera included in the low-level light imaging system, the phantom device comprising a body including an optically selective material designed to at least partially resemble the optical behavior of mammalian tissue;

instructions for reconstructing a digital representation of the light source internal to the phantom device using data included in the image; and

instructions for comparing a component of the digital representation to a known property for the light source or the phantom device.

45. The computer readable medium of claim 44 wherein the known property for the light source comprises one of:

intensity for the light source, a size of the light source, and a spectral pattern for the light source.

46. The computer readable medium of claim 44 wherein the known property for the phantom device comprises one of:

a surface size for a surface of the phantom device, a location of the light source within the phantom device, and a spectral response for the optically selective material.

47. The computer readable medium of claim 44 wherein the image comprises a luminescent image.