Methods for identifying a peptide that binds a geometrical shape
First Claim
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1. A method for identifying a peptide that binds to a surface having a target geometrical shape, comprising:
- b) contacting the surface having the target geometrical shape, with a phage display library under reaction conditions, wherein the phage express a peptide; and
c) identifying peptides that bind to the surface having the target geometrical shape.
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Abstract
Provided herein are methods, such as phage display assays, for bioengineering peptides that bind to geometrically and/or atomically structured molecular surfaces such as, for example, flat surfaces, smooth curved surfaces, as well as surfaces with periodic, random, or fractal atomic configurations. Surface-binding peptides are provided that are identified using the phage-display methods. Furthermore, scanning probe microscopy (SPM) substrates, biosensors, biochips, and electrodes are provided that include the surface-binding peptides.
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31 Claims
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1. A method for identifying a peptide that binds to a surface having a target geometrical shape, comprising:
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b) contacting the surface having the target geometrical shape, with a phage display library under reaction conditions, wherein the phage express a peptide; and
c) identifying peptides that bind to the surface having the target geometrical shape. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 24, 28, 31)
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- 20. An isolated peptide or polypeptide comprising at least two peptide units, wherein each peptide unit specifically binds a target geometrical shape, and wherein the isolated peptide or polypeptide is a recombinant peptide or polypeptide.
- 25. A scanning probe microscopy (SPM) substrate, comprising a substrate comprising an SPM material, wherein the substrate has a flat surface comprising an isolated peptide or polypeptide bound thereto.
- 29. A graphite or carbide electrode having a flat surface or a smooth, curved surface, wherein a peptide is specifically bound to the surface.
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