Mixtures of isobarically labeled analytes and fragments ions derived therefrom

• US 20050147985A1
• Filed: 04/12/2004
• Published: 07/07/2005
• Est. Priority Date: 01/05/2004
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Coull, James M., Pappin, Darryl J. C., Purkayastha, Subhasish
• Application Number:
  US10/822,639
• Publication Number:
  US 20050147985A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07B 59/00 Introduction of isotopes of elements into organic compounds ; Labelled organic compounds per se
• CPC Class Codes: C07D 295/037 with quaternary ring nitrogen atoms
• CPC Class Codes: C07D 295/108 to an acyclic saturated chain
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: G01N 33/00 Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00

Abstract

This invention pertains to mixtures of isobarically labeled analytes and fragment ions thereof.

Claims

1. A mixture comprising the same analyte labeled with two or more different isobaric labels, wherein at least two of the labeled analytes are compounds of the formula selected from the group consisting of:

2. The mixture of claim 1, wherein the analyte is a peptide.

3. The mixture of claim 1, wherein the analyte is a protein.

4. The mixture of claim 1, wherein the analyte is a nucleic acid.

5. The mixture of claim 1, wherein the analyte is a carbohydrate, lipid or steroid.

6. The mixture of claim 1, wherein the analyte is a small molecule with a molecular weight of less than 1500 daltons.

7. A mixture of fragment ions of the same analyte labeled with two or more different isobaric labels selected for fragmentation and further analysis in a tandem mass spectrometer, wherein at least two of the labeled analytes are compounds of a formula selected from the group consisting of:

8. The mixture of claim 7, wherein the analyte is a peptide.

9. The mixture of claim 7, wherein the analyte is a protein.

10. The mixture of claim 7, wherein the analyte is a nucleic acid.

11. The mixture of claim 7, wherein the analyte is a carbohydrate, lipid or steroid.

12. The mixture of claim 7, wherein the analyte is a small molecule with a molecular weight of less than 1500 daltons.

13. The mixture of claim 7, wherein the fragmentation and further analysis produces at least two signature ions of the molecular formula selected from the group consisting of:

¹³CC₅H₁₃N₂³⁰, ¹³CC₅H₁₃¹⁵NN⁺, ¹³C₂C₄H₁₃¹⁵NN⁺ and ¹³C₃C₃H₁₃¹⁵NN⁺.