Sequence of surface immobilized polymers utilizing microfluorescence detection
First Claim
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1. A method for determining the positions of polymers which terminate with a given monomer, where said polymers are attached to a surface having a plurality of positionally distinct polymers attached thereto, said method comprising the steps of:
- labeling a terminal monomer in a monomer type specific manner; and
scanning said surface, thereby determining the positions of said label.
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Abstract
Means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules. Apparatus and methods may use fluorescent labels in repetitive chemistry to determine terminal manomers on solid phase immobilized polymers. Reagents which specifically recognize terminal manomers are used to label polymers at defined positions on a solid substrate.
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Citations
11 Claims
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1. A method for determining the positions of polymers which terminate with a given monomer, where said polymers are attached to a surface having a plurality of positionally distinct polymers attached thereto, said method comprising the steps of:
- labeling a terminal monomer in a monomer type specific manner; and
scanning said surface, thereby determining the positions of said label. - View Dependent Claims (2, 3)
- labeling a terminal monomer in a monomer type specific manner; and
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4. A method for concurrently determining which subset of a plurality of positionally distinct polymers attached to a solid substrate at separable locations terminates with a given terminal subunit, said method comprising the steps of:
- mixing said solid substrate with a solution comprising a reagent, which selectively marks positionally distinct polymers which terminate with said given terminal subunit; and
determining with a detector which separable locations are marked, thereby determining which subset of said positionally distinct polymers terminated with said given terminal subunit. - View Dependent Claims (5, 6)
- mixing said solid substrate with a solution comprising a reagent, which selectively marks positionally distinct polymers which terminate with said given terminal subunit; and
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7. A method for determining which subset of a plurality of primer polynucleotides have a predetermined oligonucleotide, wherein the polynucleotides are complementary to distinctly positioned template strands which are attached to a solid substrate, said method comprising the steps of:
- selectively marking said subset of primer polynucleotides having the predetermined oligonucleotide; and
detecting which polynucleotides are marked. - View Dependent Claims (8, 9, 10)
- selectively marking said subset of primer polynucleotides having the predetermined oligonucleotide; and
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11-33. -33. (canceled)
Specification