Methods of correcting a luminescence value, and methods of determining a corrected analyte concentration
First Claim
1. A device comprising at least one binding protein having at least one reporter group attached thereto;
- and a thermometer.
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Abstract
The invention is directed to methods of correcting a luminescence value, and methods of determining a corrected analyte concentration, by use of a device capable of providing a signal when a binding protein binds to at least one analyte, and a thermometer. The invention is also directed to systems which include such a device, and a processor for correcting measured luminescence of a reporter group based on a measured temperature. The invention is further directed to apparatuses that include a memory for storing luminescence information and temperature information, and a processor for correcting luminescence information. The invention is further directed to computer programs for executing the methods of the invention, and machine-readable storage medium on which programs are recorded.
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Citations
33 Claims
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1. A device comprising
at least one binding protein having at least one reporter group attached thereto; - and
a thermometer. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A device comprising
an optical sensor capable of measuring at least one analyte in a biological sample in proximity to the optical sensor; - and
a thermometer capable of measuring a temperature of said biological sample in proximity to the optical sensor.
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8. A device comprising
at least one binding protein having at least one reporter group attached thereto; wherein the at least one reporter group is capable of detecting a temperature of a biological sample in proximity to the at least one binding protein.
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9. A method comprising
acquiring luminescence information of at least one reporter group; -
acquiring temperature information of a biological sample; and
determining a corrected luminescence value based on the temperature information;
wherein the corrected luminescence value is indicative of a concentration of at least one analyte in the sample. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 30, 31)
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22. A method comprising
using temperature information from a biological sample in proximity to at least one binding protein, said protein having at least one reporter group attached thereto, to correct an initially determined concentration of at least one analyte in the biological sample; wherein the initially-determined concentration of the at least one analyte is determined by the at least one reporter group providing a signal when the at least one binding protein binds to the at least one analyte.
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23. A method of correcting fluorescence information comprising:
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measuring fluorescence of a reporter group at multiple temperatures;
determining a temperature-to-signal relationship; and
determining at least one correction factor.
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24. A system comprising
at least one binding protein having at least one reporter group attached thereto; -
a fluorometer;
a thermometer; and
a processor. - View Dependent Claims (25)
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26. A system comprising
a sensor capable of detecting concentration of at least one analyte in a biological sample and capable of measuring temperature of the biological sample; - and
a processor. - View Dependent Claims (27)
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28. An apparatus comprising
a memory for storing luminescence information of a reporter group and temperature information; - and
a processor for correcting luminescence information based on temperature information. - View Dependent Claims (29)
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32. A machine-readable medium including instructions, execution of which by a machine determines a corrected luminescence value, the machine-readable instructions comprising:
- a code segment for determining the corrected luminescence value as a function of at least one variable selected from the group consisting of;
luminescence (L(T)) of a reporter group at temperature (T);
temperature (T);
room temperature (TR);
skin temperature (TS);
temperature sensitivity (S1) around a skin or body temperature region; and
sensitivity (S2) at a region between room temperature (TR) and skin temperature (TS).
- a code segment for determining the corrected luminescence value as a function of at least one variable selected from the group consisting of;
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33. A computer data signal embodied in a transmission medium, comprising:
a computer-readable program code, said program code comprising a code segment for determining the corrected luminescence value as a function of at least one variable selected from the group consisting of;
luminescence (L(T)) of a reporter group at temperature (T);
temperature (T);
room temperature (TR);
skin temperature (TS);
temperature sensitivity (S1) around a skin or body temperature region; and
sensitivity (S2) at a region between room temperature (TR) and skin temperature (TS).
Specification