Disposable reaction vessel with integrated optical elements

• US 20050148063A1
• Filed: 12/24/2003
• Published: 07/07/2005
• Est. Priority Date: 12/24/2003
• Status: Abandoned Application

• Current Assignee: Axela Inc.
• Original Assignee: Axela Inc.
• Inventors: Liederman, Adam Brian, Ganske, Rocky, Cracauer, Raymond Francis
• Application Number:
  US10/744,954
• Publication Number:
  US 20050148063A1
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 2021/0321 One time use cells, e.g. integrally moulded
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205

Abstract

The present invention provides disposable, semi-reusable, or single use reaction vessels with integrated optical elements for use with diffraction based assay systems. The vessel for assaying liquids for analytes includes a housing having at least one chamber or well for receiving a liquid therein and an optical element integrally formed with the housing for directing an incident light beam towards the well or chamber and directing a light beam away from the chamber after the light beam has interacted with analytes present in the liquid. The vessel may be test tube such as a blood collection tube, with or without, an optical element but having a pattern of analyte-specific receptors located on an inner surface of the tube wall so that when a liquid is introduced into the interior of the test tube analytes present in the liquid can bind with the pattern of analyte-specific receptors.

Claims

1. A vessel for assaying liquids for analytes, comprising:

a housing portion including at least one chamber for receiving a liquid therein; and

at least one optical element integrally formed with the housing portion for directing an incident light beam towards the at least one chamber and directing a light beam away from the at least one chamber after the light beam has interacted with analytes present in the liquid.

2. The vessel according to claim 1 including a pre-selected pattern of analyte-specific receptors located on an inner surface of the at least one chamber so that when a liquid is introduced into the chamber analytes present in the liquid can bind with the pattern of analyte-specific receptors, and wherein the light beam that has interacted with the pre-selected pattern of analyte-specific receptors and analytes bound thereto is a diffracted light beam.

3. The vessel according to claim 1 wherein the housing portion having at least one chamber is a standard micro-titer plate having ninety-six (96) chambers.

4. The vessel according to claim 3 including a pre-selected pattern of analyte-specific receptors located on an inner surface of the ninety-six (96) chambers so that when a liquid is introduced into the chamber analytes present in the liquid can bind with the pattern of analyte-specific receptors.

5. The vessel according to claim 1 wherein the optical element integrally formed with the housing portion is a triangular shaped optical element.

6. The vessel according to claim 2 wherein the optical element integrally formed with the housing portion is a triangular shaped optical element located below the at least one chamber, and wherein the pre-selected pattern of analyte-specific receptors are located on a bottom surface of the at least one chamber.

7. The vessel according to claim 3 wherein the ninety-six (96) chambers are arranged in rows and columns, and wherein the optical element integrally formed with the housing portion is an elongate triangular shaped optical element located below each column or row of chambers so that a total number of elongate triangular shaped optical elements is equal to the number of columns or rows in the vessel.

8. The vessel according to claim 7 including a pre-selected pattern of analyte-specific receptors located on a bottom surface of each of the ninety-six (96) chambers so that when a liquid is introduced into the chamber analytes present in the liquid can bind with the pattern of analyte-specific receptors, and wherein the light beam that has interacted with material and is a diffracted light beam.

9. The vessel according to claim 2 wherein the optical element integrally formed with the housing portion is a hemispherical-shaped optical element located below the at least one chamber, and wherein the pre-selected pattern of analyte-specific receptors are located on a bottom surface of the at least one chamber.

10. The vessel according to claim 2 wherein the optical element integrally formed with the housing portion is a conically shaped optical element located below the at least one chamber, and wherein the pre-selected pattern of analyte-specific receptors are located on a bottom surface of the at least one chamber.

11. The vessel according to claim 1 wherein the housing portion having at least one chamber includes an array of chambers for holding a plurality of liquid samples separate from each other.

12. The vessel according to claim 1 wherein the housing includes an elongate housing section and wherein the at least one chamber is an elongate chamber defined by the elongate housing section, and wherein the housing includes a cover section having a liquid inlet and a liquid outlet, which, when assembled with the elongate housing section produces a capillary flow path between the liquid inlet and liquid outlet through the elongate housing section.

13. The vessel according to claim 12 including at least one pre-selected pattern of analyte-specific receptors located along a bottom of the elongate chamber so that when a liquid is introduced into the chamber analytes present in the liquid can bind with the at least one pattern of analyte-specific receptors, and wherein the light beam that has interacted with material and substrate is a diffracted light beam.

14. The vessel according to claim 13 wherein the optical element integrally formed with the substrate is an elongate triangular shaped optical element located below the elongate chamber extending along a length of the elongate chamber.

15. The vessel according to claim 1 wherein the housing includes a generally circular substrate, and wherein the at least one chamber for receiving a liquid therein is a first chamber disposed in a center of the circular substrate, including a plurality of chambers radially displaced from the first chamber with each of the plurality of chambers being in flow communication with the first chamber through an associated flow passageway connecting each of the plurality of chambers with the first chamber, and wherein the at least one optical element includes an associated optical element located below each of the plurality of chambers.

16. The vessel according to claim 15 including a pre-selected pattern of analyte-specific receptors located on a bottom surface of each of the plurality of chambers so that when a liquid is introduced into the chamber analytes present in the liquid can bind with the pattern of analyte-specific receptors, and wherein the light beam that has interacted with material and substrate is a diffracted light beam.

17. The vessel according to claim 15 wherein the housing includes a mount for mounting the vessel on a rotational drive mechanism for spinning the vessel.

18. The vessel according to claim 1 made of molded plastic.

19. The vessel according to claim 2 wherein the at least one optical element integrally formed with the housing is located with respect to the inner surface on which the pre-selected pattern is present in order so that light directed by the at least one optical element undergoes total internal reflection.

20. The vessel according to claim 8 wherein the elongate triangular shaped optical elements are located with respect to the bottom surface of the chambers of the associated row of chambers so that light directed by the elongate triangular shaped optical elements undergoes total internal reflection.

21. The vessel according to claim 1 wherein the optical element integrally formed with the housing portion is a hemispherical-shaped optical element.

22. The vessel according to claim 1 wherein the optical element integrally formed with the housing portion is a conical-shaped optical element.

23. A vessel for assaying liquids for analytes using light diffraction, comprising:

a housing portion including at least one chamber in a top surface thereof for receiving a liquid therein; and

a pre-selected pattern of analyte-specific receptors located on an inner surface of the at least one chamber so that when a liquid is introduced into the interior of the at least one chamber analytes present in the liquid can bind with the pattern of analyte-specific receptors, wherein when analytes bind with the pre-selected pattern of analyte-specific receptors a light beam incident on the pre-selected pattern of analyte-specific receptors is diffracted.

24. The vessel according to claim 23 including an optical element integrally formed with the housing portion for directing an incident light beam towards the well and directing a diffracted light beam away from the well after the light beam has interacted with the material and substrate.

25. The vessel according to claim 23 wherein the housing portion having at least one chamber is a standard micro-titer plate having ninety-six (96) chambers.

26. The vessel according to claim 23 including an optical element integrally formed with the housing portion located below each of the ninety-six (96) chambers for directing an incident light beam towards each chamber and directing a diffracted light beam away from the chamber after the light beam has interacted with the pre-selected pattern of analyte-specific receptors and analytes bound thereto.

27. The vessel according to claim 26 wherein each of the ninety-six (96) chambers includes a pre-selected pattern of analyte-specific receptors located on a bottom surface of the at least one chamber.

28. The vessel according to claim 27 wherein the ninety-six (96) chambers are arranged in rows and columns, and wherein the optical element integrally formed with the substrate is an elongate triangular shaped optical element located below each column or row of chambers so that a total number of elongate triangular shaped optical elements is equal to the number of columns or rows in the vessel.

29. A test tube, comprising;

a cylindrical tube having a tube wall enclosing an interior and one closed end and one open end for receiving liquid into the interior of the cylindrical tube; and

a pre-selected pattern of analyte-specific receptors located on an inner surface of the tube wall so that when a liquid is introduced into the interior of the test tube analytes present in the liquid can bind with the pattern of analyte-specific receptors.

30. The test tube according to claim 29 where the test tube is a blood collection tube.

31. The test tube according to claim 29 where the test tube includes an optical element integrally formed therewith for directing an incident light beam towards the interior of the test tube and directing a light beam away from the interior of the test tube.

32. A test tube, comprising;

a cylindrical tube having a tube wall enclosing an interior and one closed end and one open end for receiving liquid into the interior of the cylindrical tube;

a pre-selected pattern of analyte-specific receptors located on an inner surface of the tube wall so that when a liquid is introduced into the interior of the test tube analytes present in the liquid can bind with the pattern of analyte-specific receptors; and

at least one optical element integrally formed with the test tube wall for directing an incident light beam towards the at least one chamber and directing a light beam away from the at least one chamber after the light beam has interacted with analytes present in the liquid.

33. The test tube according to claim 32 where the test tube is a blood collection tube.