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Human liver progenitors

  • US 20050148072A1
  • Filed: 09/21/2004
  • Published: 07/07/2005
  • Est. Priority Date: 01/19/1999
  • Status: Abandoned Application
First Claim
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49. A method of preparing a composition comprising a mixture of cells derived from human liver tissue, which mixture comprises an enriched population of human liver progenitors, the method comprising:

  • (a) providing a cell suspension of human liver tissue comprising a mixture of cells of varying sizes, including immature cells and mature cells; and

    (b) debulking the suspension based on cell size, buoyant density, or a combination thereof to remove mature cells, while retaining immature cells, to provide a mixture of cells comprised of an enriched population of human liver progenitors.

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