Induction of hepatocyte proliferation in vitro by inhibition of cell cycle inhibitors
First Claim
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1. A method of inducing proliferative and differentiative mammalian hepatocytes in vitro comprising (a) contacting the hepatocytes with protein kinase A (PKA) inhibitor.
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Abstract
The present invention provides methods of inducing proliferative and differentiative mammalian hepatocytes, or survival of differentiative mammalian hepatocytes, in vitro comprising contacting the hepatocytes with protein kinase A (PKA) inhibitor.
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45 Claims
- 1. A method of inducing proliferative and differentiative mammalian hepatocytes in vitro comprising (a) contacting the hepatocytes with protein kinase A (PKA) inhibitor.
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21. A method of promoting survival of differentiative mammalian hepatocytes in vitro comprising contacting the hepatocytes with protein kinase A inhibitor.
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22. A method of screening a compound for its effect on hepatocytes or a hepatocyte activity, comprising:
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(a) combining the compound with a cell population obtained by treating hepatocytes with a protein kinase A;
(b) determining any change to cells in the population or their activity that results from being combined with the compound; and
(c) correlating the change with the effect of the compound on hepatocytes or a hepatocyte. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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- 38. A bioartificial liver device comprising a proliferative and differentiative mammalian hepatocyte.
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43. An artificial liver device comprising:
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(a) a cell culture layer comprising cells from an isolated cell line of normal hepatocytes and a protein kinase A inhibitor, and (b) a support matrix that provides means for fluid circulation across the cell culture layer. - View Dependent Claims (44)
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45. A method of transplanting hepatocytes comprising:
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(a) inducing a population of proliferative and differentiative mammalian hepatocytes in vitro comprising contacting the hepatocytes with protein kinase A inhibitor; and
(b) transplanting the population of hepatocytes.
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Specification