×

Isobarically labeled analytes and fragment ions derived therefrom

  • US 20050148087A1
  • Filed: 05/24/2004
  • Published: 07/07/2005
  • Est. Priority Date: 01/05/2004
  • Status: Abandoned Application
First Claim
Patent Images

1. A method comprising:

  • a) reacting two or more samples, each sample comprising one or more reactive analytes, with a different labeling reagent of a set of labeling reagents to thereby produce two or more differentially labeled samples each comprising one or more labeled analytes wherein the different labeling reagents of the set each comprise the formula;


    RP-X-LK-Y-RG or a salt form and/or hydrate form thereof, wherein;

    i) RG is a reactive group that is a nucleophile or an electrophile and that is capable of reacting with one or more of the reactive analytes of the sample;

    ii) RP is a reporter moiety that comprises a fixed charge or that is ionizable, wherein the gross mass of each reporter is different for each reagent of the set;

    iii) LK is a linker moiety that links the reactive group and the reporter group, wherein the mass of the linker compensates for the difference in gross mass between the reporters for the different labeling reagents of the set such that the aggregate gross mass of the reporter and linker combination is the same for each reagent of the set;

    iv) X is a bond between an atom of the reporter and an atom of the linker;

    v) Y is a bond between an atom of the linker and an atom of the reactive group, wherein, once the labeling reagent is reacted with the reactive analyte, bond Y links the linker to the analyte; and

    vi) bonds X and Y fragment in at least a portion of the labeled analytes when subjected to dissociative energy levels;

    b) mixing two or more of the differentially labeled samples, or a portion thereof, and optionally one or more calibration standards to thereby produce a sample mixture;

    wherein RP;

    i) has a gross mass of less than 250 daltons; and

    /or ii) does not substantially sub-fragment under conditions of dissociative energy applied to cause fragmentation of at least a portion of both bonds X and Y of a labeled analyte in a mass spectrometer; and

    /or iii) is not a polymer or is not a biological polymer; and

    c) digesting each differentially labeled sample with at least one enzyme to partially, or fully, degrade components of the sample, after performing step (a).

View all claims
  • 12 Assignments
Timeline View
Assignment View
    ×
    ×