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Normalisation of microarray data based on hybridisation with an internal reference

  • US 20050153290A1
  • Filed: 12/17/2002
  • Published: 07/14/2005
  • Est. Priority Date: 12/21/2001
  • Status: Abandoned Application
First Claim
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1. A method for the identification of an analyte in a biological sample comprising the steps of:

  • (a) providing a microarray comprising a substrate with predefined regions wherein each binding substance immobilized at a predefined region onto said substrate comprises a predetermined amount of receptor and a predetermined amount of an internal reference, (b) providing a reporter molecule that binds selectively to said internal reference, (c) adding said reporter molecule to said biological sample, (d) contacting said biological sample comprising said reporter molecule with said microarray under conditions that allow binding to take place between said receptor and said analyte, and between said internal reference and said reporter molecule, (e) determining the signal of said reporter molecule binding to said internal reference, (f) determining the signal of said analyte binding to said receptor, and (g) normalising said signal of step (f) for said signal of step (e), whereby said analyte is identified.

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