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Methods for quantitative determination of methylation density in a DNA locus

  • US 20050153316A1
  • Filed: 10/21/2004
  • Published: 07/14/2005
  • Est. Priority Date: 10/21/2003
  • Status: Active Grant
First Claim
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1. A method for quantifying average methylation density in a locus of genomic DNA, the method comprising, contacting genomic DNA with a methylation-dependent restriction enzyme or a methylation-sensitive restriction enzyme under conditions that allow for at least some copies of potential restriction enzyme cleavage sites in the locus to remain uncleaved;

  • quantifying intact copies of the locus; and

    comparing the quantity of amplified product to a control value representing the quantity of methylation of control DNA, thereby quantifying the average methylation density in the locus compared to the methylation density of the control DNA.

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