Microarray method of genotyping multiple samples at multiple loci
First Claim
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1. A method of simultaneously genotyping multiple samples, the method comprising:
- amplifying genomic segments from a plurality of samples using polymerase chain reaction primers, each genomic segment comprising a genetic locus;
forming a microarray on a surface wherein material at each location on the surface corresponds essentially to a single genomic segment from a single sample;
hybridizing the microarray with a mixture of synthetic oligonucleotides, wherein the mixture comprises oligonucleotides complementary to the genomic segments; and
deriving genotyping information for multiple samples simultaneously by detecting signals from the hybridized microarray.
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Abstract
A method for genotyping multiple samples at multiple genetic loci in a single assay is provided. Microarrays of genomic segments representing discrete loci are formed and hybridized with mixtures of synthetic oligonucleotides that are complementary to the genomic segments. Genotyping information is derived by reading the microarray signals. The method can be used to characterize samples from diverse biological sources and for a variety of applications.
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24 Claims
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1. A method of simultaneously genotyping multiple samples, the method comprising:
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amplifying genomic segments from a plurality of samples using polymerase chain reaction primers, each genomic segment comprising a genetic locus;
forming a microarray on a surface wherein material at each location on the surface corresponds essentially to a single genomic segment from a single sample;
hybridizing the microarray with a mixture of synthetic oligonucleotides, wherein the mixture comprises oligonucleotides complementary to the genomic segments; and
deriving genotyping information for multiple samples simultaneously by detecting signals from the hybridized microarray. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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