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Microarray method of genotyping multiple samples at multiple loci

  • US 20050153318A1
  • Filed: 11/05/2004
  • Published: 07/14/2005
  • Est. Priority Date: 07/10/2000
  • Status: Abandoned Application
First Claim
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1. A method of simultaneously genotyping multiple samples, the method comprising:

  • amplifying genomic segments from a plurality of samples using polymerase chain reaction primers, each genomic segment comprising a genetic locus;

    forming a microarray on a surface wherein material at each location on the surface corresponds essentially to a single genomic segment from a single sample;

    hybridizing the microarray with a mixture of synthetic oligonucleotides, wherein the mixture comprises oligonucleotides complementary to the genomic segments; and

    deriving genotyping information for multiple samples simultaneously by detecting signals from the hybridized microarray.

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