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Single base extension

  • US 20050153320A1
  • Filed: 11/08/2004
  • Published: 07/14/2005
  • Est. Priority Date: 11/06/2003
  • Status: Abandoned Application
First Claim
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1. A method of detecting a genetic polymorphism, the method comprising:

  • attaching a capture molecule to a solid support in at least one solution channel, wherein the at least one solution channel is in a flowcell and the solution channel is in close proximity with a waveguide;

    injecting a sample, a polymerase, and a fluorescently labeled nucleotide into the at least one solution channel, under conditions wherein a single stranded analyte molecule present in the sample is capable of hybridizing to the capture molecule;

    hybridizing the single stranded analyte molecule to the capture molecule;

    extending the hybridized capture molecule by covalently adding a fluorescently labeled nucleotide to the hybridized capture molecule; and

    detecting the presence or absence of the covalently added fluorescently labeled nucleotide.

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