Image processing method for biochemical test

• US 20050153356A1
• Filed: 03/09/2005
• Published: 07/14/2005
• Est. Priority Date: 09/09/2002
• Status: Abandoned Application

• Current Assignee: Olympus Corporation
• Original Assignee: Olympus Corporation
• Inventors: Sato, Takatomo, Okawa, Kaneyasu
• Application Number:
  US11/076,333
• Publication Number:
  US 20050153356A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

An image processing method for a biochemical test of the present invention shoots a light image as a sample image by applying excitation illumination to an array for the biochemical test, extracts, as a reference image, a background image obtained by excluding an image of a micro pattern indicating fluorescence light generated from each probe array element from the sample image, and corrects the sample image by use of the reference image.

Claims

1. An image processing method for a biochemical test of supplying a solution of a biochemical material to an array for the biochemical test, on whose surface each biochemical material specifically reactive with the biochemical material is held, and detecting emission intensity of a luminescent molecule using a label by the luminescent molecule for each probe array element of the array for the biochemical test by an array type detection unit, whereby reaction states of the biochemical material and the biochemical material specifically reactive with the biochemical material are inspected for each of the probe array elements, the method comprising:

shooting an image of the array for the biochemical test as a sample image;

extracting, as a reference image, a background image obtained by excluding an image of a micro pattern indicating intensity of light generated from the probe array element from the sample image; and

correcting the sample image by use of the reference image.

2. The image processing method for the biochemical test according to claim 1, further comprising:

subjecting the image of the micro pattern to an eroding process and thereafter a dilating process to thereby extract the reference image.

3. The image processing method for the biochemical test according to claim 2, further comprising:

executing the eroding and dilating processes using a kernel having a predetermined shape as many as times necessary to eliminate the micro pattern.

4. The image processing method for the biochemical test according to claim 3, wherein the eroding and dilating processes comprise:

using a cross-shaped kernel of 3×

3 pixels, and setting a pixel number corresponding to a maximum radius or more of a circumscribed circle of the micro pattern as the number of process times.

5. The image processing method for the biochemical test according to claim 3, wherein the eroding and dilating processes comprise:

using a circular kernel of “

a pixel number corresponding to a maximum diameter or more of a circumscribed circle of the micro pattern”

×

“

a pixel number corresponding to the maximum diameter or more of the circumscribed circle”

pixels, and setting the number of process times to 1.

6. The image processing method for the biochemical test according to claim 1, further comprising:

dividing a luminance value of each pixel of the sample image by that of each pixel of the corresponding reference image to thereby correct the sample image.

7. The image processing method for the biochemical test according to claim 1, further comprising:

subtracting a luminance value of each pixel of the corresponding reference image from that of each pixel of the sample image to thereby correct the sample image.

8. The image processing method for the biochemical test according to claim 1, further comprising:

calculating (a maximum luminance value of the reference image/a luminance value of the pixel) with respect to each pixel of the reference image, and multiplying a luminance value of each pixel of the corresponding sample image by the calculated value to thereby correct the sample image.

9. The image processing method for the biochemical test according to claim 1, further comprising:

subjecting the reference image to a smoothing process to thereby correct the sample image.

10. The image processing method for the biochemical test according to claim 1, wherein the image of the micro pattern is a fluorescence image obtained by illuminating the array for the biochemical test having a large number of micro spots labeled with fluorescence with excitation light, and the sample image is corrected to thereby detect a signal indicating a fluorescence luminance of each micro spot.