Nucleic acid detecting cassette, nucleic and detecting apparatus utilizing nucleic acid detecting cassette, and nucleic acid detecting system utilizing nucleic acid detecting cassette
First Claim
1. A nucleic acid detecting cassette for detecting nucleic acid contained in a sample, comprising:
- a stationary member;
a flexible member provided on the stationary member, the stationary member and flexible member forming a fluid holding channel, a joining channel and an inlet-outlet port therebetween, the fluid holding channel being configured to be able to vary its inner volume, and hold a reagent, the inlet-outlet port being connected to the fluid holding channel and being selectively set to one of an open-state under which the fluid holding channel is communicated with the outside of the cassette through the port and a closed-state under which the fluid holding channel is discommunicated with the outside of the cassette, the joining channel being connected to the fluid holding channel and being selectively set to one of an open-state under which the communication with the fluid holding channel is made and a closed-state under which the communication with the fluid holding channel is interrupted;
a first opening-closing part configured to maintain the inlet-outlet port under the closed-state; and
a second opening-closing part configured to maintain the joining channel under the closed-state.
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Accused Products
Abstract
A nucleic acid detecting cassette comprises a fluid holding channel capable of varying the inner volume and holding a reagent, an inlet-outlet port connected to the fluid holding channel and capable of selecting an opened state under which the fluid injection out of the outer portion of the cassette can be achieved and a closed state under which the fluid injection can be interrupted, joining channels connected to the fluid holding channel and capable of selecting an opened state under which the fluid transfer to the other fluid holding channel can be achieved and a closed state under which the fluid transfer can be interrupted, inlet-outlet pads capable of maintaining the inlet-outlet port under a closed state, and joining pads capable of maintaining the joining channels under a closed state.
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Citations
22 Claims
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1. A nucleic acid detecting cassette for detecting nucleic acid contained in a sample, comprising:
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a stationary member;
a flexible member provided on the stationary member, the stationary member and flexible member forming a fluid holding channel, a joining channel and an inlet-outlet port therebetween, the fluid holding channel being configured to be able to vary its inner volume, and hold a reagent, the inlet-outlet port being connected to the fluid holding channel and being selectively set to one of an open-state under which the fluid holding channel is communicated with the outside of the cassette through the port and a closed-state under which the fluid holding channel is discommunicated with the outside of the cassette, the joining channel being connected to the fluid holding channel and being selectively set to one of an open-state under which the communication with the fluid holding channel is made and a closed-state under which the communication with the fluid holding channel is interrupted;
a first opening-closing part configured to maintain the inlet-outlet port under the closed-state; and
a second opening-closing part configured to maintain the joining channel under the closed-state. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A nucleic acid detecting device for detecting nucleic acid contained in a sample, comprising:
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a nucleic acid detecting cassette including;
a stationary member, a flexible member provided on the stationary member, the stationary member and flexible member forming a fluid holding channel, a joining channel and an inlet-outlet port therebetween, the fluid holding channel being configured to be able to vary its inner volume, the inlet-outlet port being connected to the fluid holding channel and being selectively set to one of an open-state under which the fluid holding channel is communicated with the outside of the cassette through the port and a closed-state-under which the fluid holding channel is discommunicated with the outside of the cassette, the joining channel being connected to the fluid holding channel and being selectively set to one of an open-state under which the communication with the fluid holding channel is made and a closed-state under which the communication with the fluid holding channel is interrupted, a detecting channel being connected to the fluid holding channel via the joining channel, a first opening-closing part configured to maintain the inlet-outlet port under the closed-state, and a second opening-closing part configured to maintain the joining channel under the closed-state;
a reagent loaded in the fluid holding channel of the nucleic acid detecting cassette; and
a nucleic acid probe of a single stranded nucleic acid immobilized within the detecting channel of the nucleic acid detecting cassette and having a base sequence complementary to that of the nucleic acid to be detected. - View Dependent Claims (15, 16)
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17. A nucleic acid detecting system for detecting nucleic acid contained in a sample, using
a nucleic acid detecting device including: -
a stationary member, a flexible member provided on the stationary member, the stationary member and flexible member forming a fluid holding channel, a joining channel and an inlet-outlet port therebetween, the fluid holding channel being configured to be able to vary its inner volume, the inlet-outlet port being connected to the fluid holding channel and being selectively set to one of an open-state under which the fluid holding channel is communicated with the outside of the cassette through the port and a closed-state under which the fluid holding channel is discommunicated with the outside of the cassette, the joining channel being connected to the fluid holding channel and being selectively set to one of an open-state under which the communication with the fluid holding channel is made and a closed-state under which the communication with the fluid holding channel is interrupted, a detecting channel being connected to the fluid holding channel via the joining channel capable of immobilizing a nucleic acid probe of a single stranded nucleic acid having a base sequence complementary to that of nucleic acid that is to be detected, a first opening-closing part configured to maintain the inlet-outlet port under the closed-state, and a second opening-closing part configured to maintain the joining channel under the closed-state;
the nucleic acid detecting system comprising;
a device holding part configured to hold the device;
a first driving mechanism configured to deform a first part of the flexible member corresponding to a first region of the fluid holding channel within the device held by the device holding part so as to deform the fluid holding channel;
a second driving mechanism configured to deform a second part of the flexible member corresponding to a second region of the fluid holding channel within the device held by the device holding part so as to deform the fluid holding channel;
a third driving mechanism configured to drive the second opening-closing part; and
a temperature control part configured to control the temperature of at least one of the fluid holding channel and the detecting channel. - View Dependent Claims (18, 19, 20, 21, 22)
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Specification