Mesenchymal precursor cell and use thereof in the repair of bone defects and fractures in mammals
First Claim
1. A method of enriching mesenchymal precursor cells, the method including the step of enriching for cells based on at least two markers, said markers being either:
- a) the presence of markers specific for mesenchymal precursor cells, or b) the absence of markers specific for differentiated mesenchymal precursor cells, or c) the levels of expression of markers specific for differentiated mesenchymal cells.
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Accused Products
Abstract
A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers is provided, as well as enriched populations of mesenchymal precursor cells and compositions comprising the cells. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilising two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
94 Citations
51 Claims
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1. A method of enriching mesenchymal precursor cells, the method including the step of enriching for cells based on at least two markers, said markers being either:
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a) the presence of markers specific for mesenchymal precursor cells, or b) the absence of markers specific for differentiated mesenchymal precursor cells, or c) the levels of expression of markers specific for differentiated mesenchymal cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 37, 38, 39, 46)
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- 25. An enriched cell population wherein at least 1% of the cells are mesenchymal precursor cells that are colony forming.
- 28. An enriched cell population wherein at least 5% of the cells are mesenchymal precursor-cells that are colony forming.
- 31. An enriched cell population wherein at least 10% of the cells are mesenchymal precursor cells that are colony forming.
- 34. An enriched cell population wherein at least 40% of the cells are mesenchymal precursor cells that are colony forming.
Specification