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Methods and compositions for the differentiation of human preadipocytes into adipocytes

  • US 20050158706A1
  • Filed: 01/14/2005
  • Published: 07/21/2005
  • Est. Priority Date: 01/29/1999
  • Status: Abandoned Application
First Claim
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1. A method for determining the ability of a compound to affect the differentiation of preadipocytes to adipocytes, the method comprising:

  • a) plating isolated human preadipocytes at a density of about 25,000 to 40,000 cells/cm2 in a preadipocyte medium comprising a defined cell culture medium having or supplemented with 1.0-4.5 g/liter glucose;

    b) differentiating said human preadipocytes with a differentiation medium comprising a defined cell culture medium;

    wherein said medium comprises 1.0-4.5 g/liter glucose, a cyclic AMP inducer, a glucocorticoid or a glucocorticoid analogue, insulin or an insulin analogue, a Peroxisome Proliferator Activated Receptor gamma agonist or a retinoic acid X receptor agonist effective, and said compound in an appropriate vehicle or vehicle alone to stimulate differentiation of said human preadipocytes;

    c) determining the number or percentage of differentiated cells using said differentiation medium containing said compound from step (b);

    d) determining the number of percentage of differentiated cells in the cells using said differentiation medium containing said vehicle alone from step (b); and

    e) comparing the number or percentage of differentiated cells from steps (c) and (d).

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