Spotting device for manufacturing DNA microarray and spotting method using the same
First Claim
1. A spotting device for dropping and immobilizing a solution of biomolecules, for example, nucleic acids such as probe DNA, mRNA, and peptide nucleic acid (PNA), and proteins on a DNA microarray surface to manufacture a DNA microarray, the spotting device including:
- a first microchannel with a tube shape;
a supplying unit supplying the solution of biomolecules to the first microchannel;
a biomolecule solution droplet forming unit cross-linked to the first microchannel and forming biomolecule solution droplets with a predetermined size by periodically jetting a gas toward the biomolecule solution flowing in the first microchannel;
a second microchannel linked to the first microchannel and having a greater diameter than the first microchannel;
a cooling unit surrounding at least a part of the second microchannel to freeze the biomolecule solution droplets which pass through the second microchannel; and
a spotting unit to thaw the biomolecule solution droplets and dropping the thawed biomolecule solution droplets on a surface of the DNA microarray.
1 Assignment
0 Petitions
Accused Products
Abstract
A spotting device for manufacturing a DNA microarray and a spotting method using the same are provided. The spotting device for dropping and immobilizing a solution of biomolecules, for example nucleic acids such as probe DNA, mRNA, and peptide nucleic acid (PNA), and proteins on a DNA microarray surface to manufacture a DNA microarray, includes a first microchannel with a tube shape; a supplying unit supplying the solution of biomolecules to the first microchannel; a biomolecule solution droplet forming unit cross-linked to the first microchannel and forming biomolecule solution droplets with a predetermined size by periodically jetting a gas toward the biomolecule solution flowing in the first microchannel; a second microchannel linked to the first microchannel and having a greater diameter than the first microchannel; a cooling unit surrounding at least a part of the second microchannel to freeze the biomolecule solution droplets which pass through the second microchannel; and a spotting unit thawing the frozen biomolecule solution droplets and dropping the thawed biomolecule solution droplets on a surface of the DNA microarray. The spotting device can form spots with uniform shape, minimize an effect of temperature on biomolecules, and easily manipulate biomolecules when manufacturing a DNA microarray.
-
Citations
13 Claims
-
1. A spotting device for dropping and immobilizing a solution of biomolecules, for example, nucleic acids such as probe DNA, mRNA, and peptide nucleic acid (PNA), and proteins on a DNA microarray surface to manufacture a DNA microarray, the spotting device including:
-
a first microchannel with a tube shape;
a supplying unit supplying the solution of biomolecules to the first microchannel;
a biomolecule solution droplet forming unit cross-linked to the first microchannel and forming biomolecule solution droplets with a predetermined size by periodically jetting a gas toward the biomolecule solution flowing in the first microchannel;
a second microchannel linked to the first microchannel and having a greater diameter than the first microchannel;
a cooling unit surrounding at least a part of the second microchannel to freeze the biomolecule solution droplets which pass through the second microchannel; and
a spotting unit to thaw the biomolecule solution droplets and dropping the thawed biomolecule solution droplets on a surface of the DNA microarray. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A spotting method for dropping and immobilizing a solution of biomolecules, for example, nucleic acids such as probe DNA, mRNA, and peptide nucleic acid (PNA), and proteins on a DNA microarray surface to manufacture a DNA microarray, the spotting method including:
-
(a) allowing the biomolecule solution to flow in a first microchannel with a tube shape;
(b) periodically jetting a gas toward the biomolecule solution flowing in the first microchannel to form biomolecule solution droplets with a predetermined size;
(c) freezing the biomolecule solution droplets passing through a second microchannel linked to the first microchannel and having a greater diameter than the first microchannel and storing the frozen biomolecule solution droplets; and
(d) thawing the biomolecule solution droplets and dropping the thawed biomolecule solution droplets on the DNA microarray surface. - View Dependent Claims (10, 11, 12, 13)
-
Specification