Device and method for multiple analyte detection
First Claim
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1. A system comprising:
- a device comprising a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers, non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers; and
a fluorescence detector for detecting a fluorescent signal, the fluorescence-detector configured for optical communication with one or more of the plurality of detection chambers.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
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Citations
35 Claims
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1. A system comprising:
a device comprising a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers, non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers; and
a fluorescence detector for detecting a fluorescent signal, the fluorescence-detector configured for optical communication with one or more of the plurality of detection chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A system comprising:
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a device comprising a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers, detection reagents provided in each of the plurality of detection chambers, the detection reagents comprising a first unlabeled oligonucleotide, a second unlabeled oligonucleotide, wherein the first unlabeled oligonucleotide and the second unlabeled oligonucleotide are each effective to bind selectively to different regions of a target nucleic acid sequence, and a fluorescently labeled oligonucleotide; and
a fluorescence-detector for detecting a fluorescent signal, the fluorescence-detector configured for optical communication with one or more of the plurality of detection chambers. - View Dependent Claims (11, 12, 13, 14)
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15. A device comprising:
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a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers; and
non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23)
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24. A device comprising:
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a plurality of detection chambers;
channel means in fluid communication with each of the plurality of detection chambers; and
detection reagents provided in each of the plurality of detection chambers, the detection reagents comprising a first unlabeled oligonucleotide, a second unlabeled oligonucleotide, wherein the first unlabeled oligonucleotide and the second unlabeled oligonucleotide are each effective to bind selectively to different regions of a target nucleic acid sequence, and a fluorescently-labeled oligonucleotide. - View Dependent Claims (25, 26, 27, 28, 29)
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30. A method, comprising:
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introducing a liquid sample comprising a target nucleic acid sequence into a chamber loaded with detection reagents, the detection reagents comprising a first unlabeled oligonucleotide;
a second unlabeled oligonucleotide; and
a fluorescently-labeled oligonucleotide; and
reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide, under conditions effective to selectively bind each of the first unlabeled oligonucleotide and the second unlabeled oligonucleotide to the target nucleic acid sequence. - View Dependent Claims (31, 32, 33, 34, 35)
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Specification