Methods for culturing human embryonic stem cells
First Claim
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1. A method of culturing single undifferentiated human embryonic stem cells (HES), comprising:
- (a) obtaining a single undifferentiated HES cell;
(b) mixing the single cell with an extracellular matrix to form a mixture to encompass the cell; and
(c) inoculating the mixture onto feeder cells with a nutrient medium.
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Abstract
The present invention discloses a method for improving growth and survival of single human embryonic stem cells. The method includes the step of obtaining a single undifferentiated HES cell; mixing the single undifferentiated cell with an extracellular matrix (ECM) to encompass the cell; and inoculating the mixture onto feeder cells with a nutrient medium in a growth environment. Therefore the single cells can survive, proliferate and grow in vitro.
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Citations
13 Claims
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1. A method of culturing single undifferentiated human embryonic stem cells (HES), comprising:
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(a) obtaining a single undifferentiated HES cell;
(b) mixing the single cell with an extracellular matrix to form a mixture to encompass the cell; and
(c) inoculating the mixture onto feeder cells with a nutrient medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification