Biosensor

• US 20050161323A1
• Filed: 10/04/2002
• Published: 07/28/2005
• Est. Priority Date: 05/20/2002
• Status: Active Grant

• Current Assignee: INFOPIA CO., LTD
• Original Assignee: INFOPIA CO., LTD
• Inventors: Lee, Sung-Dong, Lee, Heon-Kwon, Kwon, Mi-Joong, Bae, Byung-Woo
• Granted Patent: US 7,070,680 B2
• Time in Patent Office: Days
• US Class Current: 204/403.10
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

Disclosed is a biosensor for quantifying a specific substance contained in a biological sample comprising: an electrically insulating base plate; a plurality of lead terminals formed on the base plate; a plurality of lead wires connected to the lead terminals, respectively; an electrode system including two working electrodes and one reference electrode connected to the lead wires, respectively; an insulating layer for insulating the electrodes; an enzyme reaction layer formed on the insulating layer and the electrodes; a spacer formed on the enzyme reaction layer so as to ensure a sufficient space for receiving a sample; and a cover formed on the spacer, wherein the spacer has a sample introduction port opened at one side of the spacer, and the cover has at least one slit for venting air existing in a sample receiving space defined by the spacer and the cover, the slit extending to above the electrodes from one end of the cover.

Claims

1. A biosensor comprising:

an electrically insulating base plate 20;

a plurality of lead terminals 32 formed on the base plate 20;

a plurality of lead wires 31 connected to the lead terminals 32, respectively;

an electrode system 40 including two working electrodes 42 and 43 and one reference electrode 41 connected to the lead wires 31, respectively;

an insulating layer 50 for insulating the electrodes 41, 42 and 43;

an enzyme reaction layer 80 formed on the insulating layer 50 and the electrodes 41, 42 and 43;

a spacer 60 formed on the enzyme reaction layer 80 so as to ensure a sufficient space for receiving a sample; and

a cover 70 formed on the spacer 60,

wherein the spacer 60 has a sample introduction port 61 opened at one side of the spacer 60, and the cover 70 has at least one slit 71 for venting air existing in a sample receiving space 62 defined by the spacer 60 and the cover 70, the slit 71 extending to above the electrodes 41, 42 and 43 from one end of the cover 70.

2. The biosensor as set forth in claim 1, further including a curved groove 72 formed at one end of the cover 70 toward the electrodes 41, 42 and 43, the slit 71 being formed from a certain position of the curved groove 72 with a predetermined length.

3. The biosensor as set forth in claim 1, wherein the slit 71 is a plurality of slits spaced at a predetermined interval along a length direction.

4. The biosensor as set forth in claim 1, wherein the respective working electrodes 42 and 43 have the same electrical resistance and area, and the reference electrode 41 is spaced at the same distance from the respective working electrodes 42 and 43.

5. The biosensor as set forth in claim 4, wherein the reference electrode 41 has an area more than 1.5 times larger than the working electrodes 42 and 43.

6. The biosensor as set forth in claim 1, wherein when the sample receiving space 62 is placed in a vertical direction, the reference electrode 41 has an H-shape, and the respective working electrodes 42 and 43 are arranged at the upper and lower portions of a horizontal axis of the reference electrode 41.

7. The biosensor as set forth in claim 6, wherein the respective working electrodes 42 and 43 have the same electrical resistance and area, and the reference electrode 41 is spaced at the same distance from the respective working electrodes 42 and 43.

8. The biosensor as set forth in claim 1, wherein when the sample receiving space 62 is placed in a vertical direction, the reference electrode 41 has an E-shape, and the respective working electrodes 42 and 43 are arranged at the upper and lower portions of a horizontal axis B, which is formed at the center of the reference electrode 41.

9. The biosensor as set forth in claim 8, wherein the respective working electrodes 42 and 43 have the same electrical resistance and area, and the reference electrode 41 is spaced at the same distance from the respective working electrodes 42 and 43.

10. The biosensor as set forth in claim 2, wherein the slit 71 is a plurality of slits spaced at a predetermined interval along a length direction.

11. The biosensor as set forth in claim 2, wherein the respective working electrodes 42 and 43 have the same electrical resistance and area, and the reference electrode 41 is spaced at the same distance from the respective working electrodes 42 and 43.

12. The biosensor as set forth in claim 11, wherein the reference electrode 41 has an area more than 1.5 times larger than the working electrodes 42 and 43.