Extraction system

• US 20050161377A1
• Filed: 01/24/2005
• Published: 07/28/2005
• Est. Priority Date: 01/23/2004
• Status: Abandoned Application

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Fujimoto, Keiichi, Torisawa, Nobuyuki
• Application Number:
  US11/039,941
• Publication Number:
  US 20050161377A1
• Time in Patent Office: Days
• US Class Current: 210/120
• CPC Class Codes: B01L 2200/146 Employing pressure sensors
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: C12N 15/1017 by filtration, e.g. using filters, frits, membranes
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 2001/4016 being a selective membrane, e.g. dialysis or osmosis

Abstract

An extraction cartridge having a filter member extracts a specific material such as a nucleic acid. The specific material is caused to be adsorbed to the filter member by pressurizing a sample liquid containing a specific material. A pressurized-air supply mechanism for introducing pressurized air into a plurality of the extraction cartridges includes an air pump, opening and closing valves for individually starting or stopping supply of the pressurized air to the plurality of extraction cartridges, and pressure relief valves for individually relieving the pressures in the extraction cartridges to the atmosphere. These valves are disposed partway along a feed passage to the extraction cartridges. The extraction cartridges are supplied with pressurized air, and then sealed. Upon completion of the drainage of any one of the extraction cartridges, the pressure relief valve associated with the completely drained extraction cartridge is independently opened to relieve the pressurized air remaining therein.

Claims

1. An extraction system for extracting a specific material, comprising:

an extraction cartridge provided with a filter member, wherein a sample liquid containing a specific material is injected into the extraction cartridge and the inside of the extraction cartridge is pressurized to cause the specific material within the sample liquid to be adsorbed to the filter member; and

a pressurized-air supply mechanism for introducing pressurized air into the extraction cartridge, the pressurized-air supply mechanism including;

an air pump;

a feed passage extending from the air pump to a plurality of extraction cartridges;

a plurality of opening and closing valves, disposed partway along the feed passage and associated with the plurality of extraction cartridges in one-to-one correspondence, for individually starting or stopping the supply of the pressurized air to the plurality of extraction cartridges; and

a plurality of pressure relief valves, disposed partway along the feed passage and associated with the plurality of extraction cartridges in one-to-one correspondence, for individually relieving the pressure within the plurality of extraction cartridges.

3. The extraction system as defined in claim 1, wherein the opening and closing valves are independently actuated to individually introduce the pressurized air into the plurality of the extraction cartridges, then the plurality of the extraction cartridges are sealed, and upon completion of the drainage of any one of the extraction cartridges, the pressure relief valve associated with the completely drained extraction cartridge is independently opened to relieve the pressurized air remaining in the extraction cartridge.

5. The extraction system as defined in claim 1, wherein the pressurized-air supply mechanism further comprises a pressure sensor for individually detecting the internal pressure of the extraction cartridge, and the pressure relief valves are individually opened depending on a detection result provided by the pressure sensor.

7. The extraction system as defined in claim 3, wherein the pressurized-air supply mechanism further comprises a pressure sensor for individually detecting the internal pressure of the extraction cartridge, and the pressure relief valves are individually opened depending on a detection result provided by the pressure sensor.

2. An extraction system for extracting a nucleic acid, comprising:

an extraction cartridge provided with a filter member, wherein a sample liquid containing a nucleic acid is injected into the extraction cartridge, the inside of the extraction cartridge is pressurized to cause the nucleic acid within the sample liquid to be adsorbed to the filter member, a recovery liquid is subsequently dispensed into the extraction cartridge, the inside of extraction cartridge is pressurized to cause the nucleic acid adsorbed to the filter member to be desorbed therefrom, and the nucleic acid is collected together with the recovery liquid; and

a pressurized-air supply mechanism for introducing pressurized air into the extraction cartridge, the pressurized-air supply mechanism including;

an air pump;

a feed passage extending from the air pump to a plurality of extraction cartridges;

a plurality of opening and closing valves, disposed partway along the feed passage and associated with the plurality of extraction cartridges in one-to-one correspondence, for individually starting or stopping supply of the pressurized air to the plurality of extraction cartridges; and

a plurality of pressure relief valves, disposed partway along the feed passage and associated with the plurality of extraction cartridges in one-to-one correspondence, for individually relieving the pressure within the plurality of extraction cartridges.

4. The extraction system as defined in claim 2, wherein the opening and closing valves are independently actuated to individually introduce the pressurized air into the plurality of the extraction cartridges, then the plurality of the extraction cartridges are sealed, and upon completion of the drainage of any one of the extraction cartridges, the pressure relief valve associated with the completely drained extraction cartridge is independently opened to relieve the pressurized air remaining in the extraction cartridge.

6. The extraction system as defined in claim 2, wherein the pressurized-air supply mechanism further comprises a pressure sensor for individually detecting the internal pressure of the extraction cartridge, and the pressure relief valves are individually opened depending on a detection result provided by the pressure sensor.

8. The extraction system as defined in claim 4, wherein the pressurized-air supply mechanism further comprises a pressure sensor for individually detecting the internal pressure of the extraction cartridge, and the pressure relief valves are individually opened depending on a detection result provided by the pressure sensor.

9. An extraction system for extracting a specific material, comprising:

an extraction cartridge provided with a filter member, wherein a sample liquid containing a specific material is injected into the extraction cartridge and the inside of the extraction cartridge is pressurized to cause the specific material within the sample liquid to be adsorbed to the filter member; and

a pressurized-air supply mechanism for introducing pressurized air into the extraction cartridge, the pressurized-air supply mechanism including;

an air pump;

means for controlling air flow rate; and

means for measuring air flow rate, wherein the means for controlling air flow rate is controlled depending on a measurement result from the means for measuring air flow rate, such that the flow rate of the air to be supplied to the extraction cartridge falls within a predetermined range.

11. The extraction system as defined in claim 9, wherein the means for controlling air flow rate is a means for regulating the discharge rate from the air pump by drive-controlling the air pump.

13. The extraction system as defined in claim 9, wherein the means for controlling air flow rate is a means for restricting the air flow rate at the suction side or discharge side of the air pump by the use of a flow rate control valve.

15. The extraction system as defined in claim 9, wherein the means for measuring air flow rate is a means for measuring a pressure in association with driving of the air pump in a closed circuit for air flow.

17. The extraction system as defined in claim 11, wherein the means for measuring air flow rate is a means for measuring a pressure in association with driving of the air pump in a closed circuit for air flow.

19. The extraction system as defined in claim 13, wherein the means for measuring air flow rate is a means for measuring a pressure in association with driving of the air pump in a closed circuit for air flow.

21. The extraction system as defined in claim 9, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

23. The extraction system as defined in claim 11, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

25. The extraction system as defined in claim 13, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

27. The extraction system as defined in claim 15, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

10. An extraction system for extracting a nucleic acid, comprising:

a plurality of extraction cartridges each provided with a filter member, wherein a sample liquid containing a nucleic acid is injected into the extraction cartridges, the inside of the extraction cartridge is pressurized to cause the nucleic acid within the sample liquid to be adsorbed to the filter member, a recovery liquid is subsequently dispensed into the extraction cartridges, the inside of the extraction cartridge is pressurized to cause the nucleic acid adsorbed to the filter member to be desorbed therefrom, and the nucleic acid is collected together with the recovery liquid; and

a pressurized-air supply mechanism for introducing pressurized air into the extraction cartridge, the pressurized-air supply mechanism including;

an air pump;

means for controlling air flow rate; and

means for measuring air flow rate, wherein the means for controlling air flow rate is controlled depending on a measurement result from the means for measuring air flow rate, such that the flow rate of the air to be supplied to the extraction cartridge falls within a predetermined range.

12. The extraction system as defined in claim 10, wherein the means for controlling air flow rate is a means for regulating the discharge rate from the air pump by drive-controlling the air pump.

14. The extraction system as defined in claim 10, wherein the means for controlling air flow rate is a means for restricting the air flow rate at the suction side or discharge side of the air pump by the use of a flow rate control valve.

16. The extraction system as defined in claim 10, wherein the means for measuring air flow rate is a means for measuring a pressure in association with driving of the air pump in a closed circuit for air flow.

18. The extraction system as defined in claim 12, wherein the means for measuring air flow rate is a means for measuring a pressure in association with driving of the air pump in a closed circuit for air flow.

20. The extraction system as defined in claim 14, wherein the means for measuring air flow rate is a means for measuring a pressure in association with driving of the air pump in a closed circuit for air flow.

22. The extraction system as defined in claim 10, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

24. The extraction system as defined in claim 12, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

26. The extraction system as defined in claim 14, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

28. The extraction system as defined in claim 16, wherein the air flow rate is corrected by driving the air pump at power-on, at the start of each extraction, or at predetermined timings.

29. An extraction system for extracting a specific material, comprising:

an extraction cartridge provided with a filter member, wherein a sample liquid containing a specific material is injected into an extraction cartridge and the inside of the extraction cartridge is pressurized to cause the specific material within the sample liquid to be adsorbed to the filter member; and

a pressurized-air supply mechanism for introducing pressurized air into the extraction cartridge, the pressurized-air supply mechanism starting to supply the pressurized air to a plurality of extraction cartridge substantially simultaneously, controlling the pressure imparted to the filter member within the cartridges at a predetermined pressure, and relieving the pressure after the drainage of the sample liquid within the cartridge is completed.