Method for detecting biochemical reactant biochip
First Claim
1. A method for detecting a biochemical reactant comprising the steps of:
- hybridizing a biochemical specimen with a nucleic acid probe on a biochip, the nucleic acid probe including a configuration of a loop structure and arrayed on one or more electrodes provided on a surface of a substrate or substrate analog or the nucleic acid probe with said configuration but further including a label modification added in advance; and
detecting/discriminating a complex of the nucleic acid probe and the biochemical specimen, forming a double chain, by means of at least one of electrical, magnetic and optical changes on the surface of the biochip.
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Accused Products
Abstract
A biochemical reactant is detected by means of a biochip with good reproducibility. A nucleic acid probe is made to have a three-dimensional structure by adopting a loop structure in such a way that a free end not fixed to the surface of a substrate or a portion with a modifiable label is located on an electrode of a biochip or near the surface of the electrode on the substrate side. Alternatively, an essential portion complementarily connected to the biochemical specimen can be located on the substrate side. As a result the nucleic acid probe has a property that it can be basically hybridized with only the object specimen. When the nucleic acid probe is hybridized with the sample specimen, the loop structure is destroyed, and the label of only the hybridized probe can be modified.
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Citations
22 Claims
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1. A method for detecting a biochemical reactant comprising the steps of:
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hybridizing a biochemical specimen with a nucleic acid probe on a biochip, the nucleic acid probe including a configuration of a loop structure and arrayed on one or more electrodes provided on a surface of a substrate or substrate analog or the nucleic acid probe with said configuration but further including a label modification added in advance; and
detecting/discriminating a complex of the nucleic acid probe and the biochemical specimen, forming a double chain, by means of at least one of electrical, magnetic and optical changes on the surface of the biochip. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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2. A method for detecting a biochemical reactant comprising the steps of:
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hybridizing a biochemical specimen with a nucleic acid probe on a biochip, the nucleic acid probe including a configuration of a loop structure and arrayed on one or more electrodes provided on a surface of a substrate or substrate analog or the nucleic acid probe with said configuration but further including a label modification added in advance;
modifying with a label either during or after hybridization one or both of the biochemical specimen and the probe nucleic acid forming a double chain; and
detecting/discriminating a complex of the probe nucleic acid and the biochemical specimen, forming a double chain, by means of at least one of electrical, magnetic and optical changes on the surface of the biochip.
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3. A method for detecting a biochemical reactant comprising the steps of:
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hybridizing a biochemical specimen modified in advance with a label with a nucleic acid probe on a biochip, the nucleic acid probe including a configuration of a loop structure and arrayed on one or two or more electrodes provided on a surface of a substrate or substrate analog or the nucleic acid probe with said configuration but further including a label modification added in advance; and
detecting/discriminating a complex of the probe nucleic acid and the biochemical specimen, forming a double chain, by means of at least one of electrical, magnetic and optical changes on the surface of the biochip.
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4. A method for detecting a biochemical reactant comprising the steps of:
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hybridizing a biochemical specimen modified in advance with a label with a nucleic acid probe on a biochip, the nucleic acid probe including a configuration of a loop structure and arrayed on one or two or more electrodes provided on a surface of a substrate or substrate analog or the nucleic acid probe with said configuration but further including a label modification added in advance;
modifying with a label either during or after hybridization one or both of the biochemical specimen and the probe nucleic acid forming a double chain; and
detecting/discriminating a complex of the probe nucleic acid the biochemical specimen, forming a double chain, by means of at least one of electrical, magnetic and optical changes on the surface of the biochip.
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15. A biochip comprising:
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a substrate or a substrate analog;
at least one electrode formed on a surface of the substrate or the substrate analog; and
a nucleic acids probe arrayed on said electrode, each nucleic acid probe having a loop structure. - View Dependent Claims (21, 22)
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16. A biochip comprising:
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a substrate or a substrate analog;
at least one electrode formed on a surface of the substrate or the substrate analog; and
a nucleic acids probe arrayed on said electrode, wherein the arrayed nucleic acids probe has a loop structure in which a principal part that binds complementarily to the biochemical specimen is located on a substrate or substrate analog side.
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17. A biochip comprising:
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a substrate or a substrate analog;
at least one electrode formed on a surface of the substrate or the substrate analog; and
a nucleic acids probe arrayed on the surface of said electrode, wherein the arrayed nucleic acids probe has a loop structure in which a free end not fixed to a surface of the electrode or a site of the loop structure capable of being modified with a label is located on a substrate or substrate analog side.
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18. A biochip comprising:
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a substrate or a substrate analog;
at least one electrode formed on a surface of the substrate or the substrate analog; and
a nucleic acid probe arrayed on said electrode, wherein the arrayed nucleic acid probe has a loop structure in which a site modified with a first label which allows further modification with a second label is located on a substrate or substrate analog side. - View Dependent Claims (20)
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19. A biochip comprising:
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a substrate or a substrate analog;
at least one electrode formed on a surface of the substrate or the substrate analog; and
a nucleic acid probe arrayed on the surface of said electrode, wherein the arrayed nucleic acid probe has a loop structure in which a label previously attached is located on a substrate or substrate analog side.
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Specification