Methods for rapid purification of nucleic acids for subsquent analysis by mass spectrometery by solution capture
First Claim
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1. A method of purifying a solution comprising a nucleic acid comprising:
- mixing said solution with an anion exchange resin to yield a suspension of said anion exchange resin in said solution wherein said nucleic acids bind to said anion exchange resin;
isolating said anion exchange resin from said solution;
washing said anion exchange resin to remove one or more contaminants with one or more wash buffers while retaining bound nucleic acids;
eluting said nucleic acids from said ion exchange resin with an elution buffer.
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Abstract
The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.
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Citations
42 Claims
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1. A method of purifying a solution comprising a nucleic acid comprising:
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mixing said solution with an anion exchange resin to yield a suspension of said anion exchange resin in said solution wherein said nucleic acids bind to said anion exchange resin;
isolating said anion exchange resin from said solution;
washing said anion exchange resin to remove one or more contaminants with one or more wash buffers while retaining bound nucleic acids;
eluting said nucleic acids from said ion exchange resin with an elution buffer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method of purifying a solution comprising a nucleic acid for subsequent analysis by electrospray mass spectrometry comprising:
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adding a weak anion exchange resin to said solution and mixing to yield a suspension of said weak anion exchange resin in said solution wherein said nucleic acids bind to said weak anion exchange resin;
isolating said weak anion exchange resin from said solution;
washing said weak anion exchange resin to remove contaminants with one or more wash buffers while retaining bound nucleic acids; and
eluting said nucleic acids from said weak ion exchange resin with an elution buffer. - View Dependent Claims (27, 28, 29)
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30. A method of purifying a solution of nucleic acids for subsequent analysis by electrospray mass spectrometry comprising:
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adding ProPac™
WAX weak anion exchange resin to said solution and mixing to yield a suspension of said weak anion exchange resin in said solution wherein said nucleic acids bind to said weak anion exchange resin;
isolating said weak anion exchange resin from said solution;
washing said weak anion exchange resin to remove contaminants with one or more wash buffers while retaining bound nucleic acids; and
eluting said nucleic acids from said weak ion exchange resin with an elution buffer comprising piperidine, imidazole and methanol. - View Dependent Claims (31)
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32. A kit for purification of nucleic acids comprising:
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a resin source plate containing anion exchange resin;
a sample mixing plate;
a filter plate or a microtube rack comprising a plurality of micro filter tubes;
one or more resin wash solutions; and
an ESI-MS-compatible elution buffer. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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Specification