Assays for the detection of microtubule depolymerization inhibitors
0 Assignments
0 Petitions
Accused Products
Abstract
This invention provides methods for the screening and identification of agents having potent effects on the progression of the cell cycle. In one embodiment, the methods involve contacting a polymerized microtubule with a microtubule severing protein or a microtubule depolymerizing protein in the presence of an ATP or a GTP and a test agent; and (ii) detecting the formation of tubulin monomers, dimers or oligomers. The p60 subunit of katanin provides a particularly preferred microtubule severing protein possessing both ATPase and microtubule severing activities.
-
Citations
74 Claims
-
1-25. -25. (canceled)
- 26. An isolated polypeptide having microtubule severing activity, said polypeptide comprising an amino acid sequence that is encoded by a nucleic acid sequence that hybridizes under stringent conditions with a nucleic acid sequence that encodes SEQ ID NO:
-
30-33. -33. (canceled)
- 34. A kit for screening for agents that modulate microtubule depolymerization, said kit comprising one or more containers containing one or more of an isolated microtubule severing protein and a microtubule depolymerizing protein.
-
40-41. -41. (canceled)
-
43-59. -59. (canceled)
-
65. An isolated polypeptide comprising SEQ ID NO:
- 1 and having microtubule severing activity.
-
66. An isolated polypeptide consisting of SEQ ID NO:
- 1.
Specification