Substrate determining method
First Claim
1. A method for quantitating a substrate in a sample solution, which contains a dissolved interfering substance and said substrate, by the use of an electrode system and a reagent system, comprising the steps of:
- (a) supplying a sample solution which contains a dissolved interfering substance and a substrate to an electrode system comprising a working electrode and a counter electrode under the existence of a reagent system comprising oxidoreductase and an electron mediator;
(b) applying an AC potential to said working electrode to cause a redox reaction of said electron mediator;
(c) measuring an electric signal produced on the basis of said redox reaction, by means of said electrode system; and
(d) quantitating said substrate on the basis of said electric signal.
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Accused Products
Abstract
There is provided a method for precisely quantitating a substrate by means of a measurement system with a simple structure without occurrence of a measurement error due to an interfering substance. In a method for quantitating a substrate in a sample solution which contains a dissolved interfering substance and the substrate, by the use of an electrode system and a reagent system, (a) a sample solution which contains a dissolved interfering substance and a substrate is supplied to an electrode system comprising a working electrode and a counter electrode under the existence of a reagent system comprising oxidoreductase and an electron mediator; (b) an AC potential is applied to the working electrode, to cause a redox reaction of the electron mediator: (c) an electric signal produced on the basis of the redox reaction is measured by means of the electrode system; and (d) the substrate is quantitated on the basis of the electric signal.
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Citations
11 Claims
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1. A method for quantitating a substrate in a sample solution, which contains a dissolved interfering substance and said substrate, by the use of an electrode system and a reagent system, comprising the steps of:
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(a) supplying a sample solution which contains a dissolved interfering substance and a substrate to an electrode system comprising a working electrode and a counter electrode under the existence of a reagent system comprising oxidoreductase and an electron mediator;
(b) applying an AC potential to said working electrode to cause a redox reaction of said electron mediator;
(c) measuring an electric signal produced on the basis of said redox reaction, by means of said electrode system; and
(d) quantitating said substrate on the basis of said electric signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification