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Stem cell culture medium and method of using said medium and the cells

  • US 20050164380A1
  • Filed: 11/04/2004
  • Published: 07/28/2005
  • Est. Priority Date: 11/04/2003
  • Status: Active Grant
First Claim
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1. A method of enriching stem cells in a plurality of cells, comprising the steps of:

  • adding a sample of cells to a first liquid cell culture medium under conditions wherein stem cells are substantially in suspension and wherein non-proliferating cells substantially adhere to a substrate; and

    passaging suspended cells from the first medium into a second liquid cell culture medium, thereby enriching the stem cells.

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