Embryonic stem cells
First Claim
1. A purified preparation of human undifferentiated embryonic stem cells capable of proliferation in vitro.
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Abstract
The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation, production of differentiated cells and in particular the production of human embryonic stem cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells capable of giving rise to mature somatic cells and uses thereof. The present invention also provides a purified preparation of undifferentiated human embryonic stem cells capable of proliferation in vitro. Furthermore, the present invention provides a somatic cell differentiated in vitro from an undifferentiated embryonic stem cell. There is also provided a committed progenitor cell capable of giving rise to mature somatic cells.
23 Citations
36 Claims
- 1. A purified preparation of human undifferentiated embryonic stem cells capable of proliferation in vitro.
- 6. An undifferentiated human embryonic stem cell wherein the cell is immunoreactive with markers for human pluripotent stem cells including SSEA-4, GCTM-2 antigen, and TRA 1-60.
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8. A method of preparing undifferentiated human embryonic stem cells, said method including:
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obtaining an in vitro fertilised human embryo and growing the embryo to a blastocyst stage of development;
removing inner cells mass (ICM) cells from the embryo;
culturing ICM cells under conditions which do not induce extraembryonic differentiation and cell death; and
promote proliferation of undifferentiated stem cells; and
recovering stem cells.
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9. A method of preparing undifferentiated human embryonic stem cells, said method including:
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obtaining an in vitro fertilised human embryo and growing the embryo to a blastocyst stage of development;
removing inner cell mass (ICM) cells from the embryo;
culturing ICM cells on a fibroblast feeder layer to obtain proliferation of undifferentiated stem cells; and
recovering stem cells from the feeder layer. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 35)
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29. A cell line HES-1.
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30. A cell line HES-2.
- 31. A fibroblast cell strain which is highly suitable for the promotion of embryonic stem cell growth and the inhibition of extraembryonic differentiation.
- 33. A method of preserving a differentiated or undifferentiated cell wherein the cells undergo vitrification.
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36. A human embryonic stem cell line as hereinbefore described with reference to the examples.
Specification