Fatty acid desaturase gene obtained from pomegranate and method for the production of unsaturated fatty acids
First Claim
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1. An isolated nucleic acid that encodes a polypeptide with a desaturase activity, wherein said nucleic acid contains a sequence selected from the group consisting of:
- the sequence of SEQ ID NO;
2;
the sequence of SEQ ID NO;
7;
a sequence derived from the amino acid sequence of SEQ ID NO;
3 or SEQ ID NO;
8 by back translation of the respective amino acid sequences, owing to the degeneracy of the genetic code; and
a derivative of the sequence of SEQ ID NO;
2 or SEQ ID NO;
7 wherein said derivative encodes a peptide that has at least 75 % identity at the amino acid level to said polypeptide, wherein the desaturase activity of the peptide is not substantially reduced as compared with the desaturase activity of said polypeptide.
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Abstract
The present invention relates to a process for the production of unsaturated or saturated fatty acids and to a process for the production of oils and/or triglycerides with an increased content of unsaturated or saturated fatty acids. The invention furthermore relates to nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the nucleic acid sequences, nucleic acid constructs and/or vectors. Moreover, the invention relates to fatty acid mixtures and to triglycerides with an increased content of unsaturated fatty acids, and to their use.
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Citations
58 Claims
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1. An isolated nucleic acid that encodes a polypeptide with a desaturase activity, wherein said nucleic acid contains a sequence selected from the group consisting of:
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the sequence of SEQ ID NO;
2;
the sequence of SEQ ID NO;
7;
a sequence derived from the amino acid sequence of SEQ ID NO;
3 or SEQ ID NO;
8 by back translation of the respective amino acid sequences, owing to the degeneracy of the genetic code; and
a derivative of the sequence of SEQ ID NO;
2 or SEQ ID NO;
7 wherein said derivative encodes a peptide that has at least 75 % identity at the amino acid level to said polypeptide, wherein the desaturase activity of the peptide is not substantially reduced as compared with the desaturase activity of said polypeptide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 31, 32)
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23. An isolated nucleic acid sequence that encodes a protein that converts a fatty acid of structure I
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24. An isolated nucleic acid that encodes a polypeptide with a desaturase activity, wherein said nucleic acid contains a sequence selected from the group consisting of:
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the sequence of SEQ ID NO;
5;
a sequence derived from the amino acid sequence of SEQ ID NO;
6 by back translation of said amino acid sequence, owing to the degeneracy of the genetic code;
a derivative of the sequence of SEQ ID NO;
5 that encodes a peptide which has at least 90% identity at the amino acid level with the amino acid sequence of SEQ ID NO;
6, wherein the desaturase activity of said peptide is not substantially reduced as compared with the desaturase activity of said polypeptide. - View Dependent Claims (25, 26, 27, 28, 29, 30)
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33. An isolated nucleic acid that encodes a polypeptide with a non-functional desaturase activity, wherein said nucleic acid contains a sequence selected from the group consisting of:
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the sequence of SEQ ID NO;
2;
the sequence of SEQ ID NO;
7;
a sequence derived from the amino acid sequence of SEQ ID NO;
3 or SEQ ID NO;
8 by back translation of the respective amino acid sequences, owing to the degeneracy of the genetic code; and
a derivative of the sequence of SEQ ID NO;
2 or SEQ ID NO;
7 wherein said derivative encodes a peptide that has at least 75 % identity at the amino acid level to said polypeptide. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 51, 52)
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46. A process for production of oils or triglycerides with an increased content of saturated fatty acids, which comprises:
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introducing a nucleic acid to an oil-producing organism wherein said nucleic acid encodes a polypeptide with a non-functional desaturase activity, wherein said nucleic acid contains a sequence selected from the group consisting of;
the sequence of SEQ ID NO;
2;
the sequence of SEQ ID NO;
7;
a sequence derived from the amino acid sequence of SEQ ID NO;
3 or SEQ ID NO;
8 by back translation of the respective amino acid sequences, owing to the degeneracy of the genetic code; and
a derivative of the sequence of SEQ ID NO;
2 or SEQ ID NO;
7wherein said derivative encodes a peptide that has at least 75 % identity at the amino acid level to said polypeptide;
culturing said organism; and
isolating said oils or triglycerides. - View Dependent Claims (47, 48, 49, 50)
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53. An isolated nucleic acid that encodes a polypeptide with a non-functional desaturase activity, wherein said nucleic acid contains a sequence selected from the group consisting of:
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the sequence of SEQ ID NO;
5;
a sequence derived from the amino acid sequence of SEQ ID NO;
6 by back translation of said amino acid sequence, owing to the degeneracy of the genetic code; and
a derivative of the sequence of SEQ ID NO;
5 that encodes a peptide which has at least 90% identity at the amino acid level with the amino acid sequence of SEQ ID NO;
6. - View Dependent Claims (54, 55, 56, 57, 58)
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Specification