Biomolecular substrate, method of testing or diagnosis with use thereof and apparatus therefor

• US 20050169797A1
• Filed: 05/07/2003
• Published: 08/04/2005
• Est. Priority Date: 05/08/2002
• Status: Active Grant

• Current Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Oshima, Mitsuaki
• Granted Patent: US 7,438,854 B2
• Time in Patent Office: Days
• US Class Current: 422/50
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00466 in a slurry
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00545 Colours
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 3/545 for laboratory containers
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

A test apparatus is for testing a DNA substrate on which a plurality of DNA fragments for testing are arranged, wherein absolute precision is not required. A substrate is provided on which a plurality of biomolecule spots containing a group of biomolecules (e.g., DNA, etc.) of a specific type are formed, where the pattern or position of the DNA spot is changed depending on specific data so that information of the specific data is recorded on the substrate.

Claims

1. A biomolecule bead-containing tube containing a biomolecule bead array in which biomolecule beads consisting of a spherical bead and a specific biomolecule species immobilized thereon are arranged in a tubular container made of a material transmitting a light having a specific wavelength, wherein a spherical mark bead made of a material optically distinguishable from the material constituting the spherical bead of said biomolecule bead is inserted in a predetermined order between specific biomolecule beads in the biomolecule bead array.

2. A biomolecule bead-containing tube according to claim 1, wherein the mark beads are arranged corresponding to an identification code indicating identification data.

3. A biomolecule bead-containing tube according to claim 1, having a first region where a number of the biomolecule beads is larger than a number of the mark beads, and a second region where a number of the mark beads is larger than a number of the biomolecule beads.

4. A biomolecule bead-containing tube according to claim 3, wherein at least the mark beads are arranged in the second region corresponding to an identification code indicating identification data.

5. A biomolecule bead-containing tube according to claim 2 or 4, wherein the identification data comprise an identification number for the biomolecule beads-containing tube.

6. A biomolecule bead-containing tube according to claim 3, wherein the mark beads are arranged in the first region corresponding to an identification code indicating identification data.

13. A biomolecule bead-containing tube according to claim 4, wherein the identification data comprise an identification number for the biomolecule beads-containing tube.

7. A reproducer reading out data recorded in a biomolecule bead-containing tube, biomolecule bead-containing tube containing a biomolecule bead array in which biomolecule beads consisting of a spherical bead and a specific biomolecules Decies immobilized thereon are arranged in a tubular container made of a material transmitting a light having a specific wavelength, wherein a spherical mark bead made of a material optically distinguishable from the material constituting the spherical bead of said biomolecule bead is inserted in a predetermined order between specific biomolecule beads in the biomolecule bead array, wherein the mark beads are arranged corresponding to an identification code indicating identification data, said data read out by irradiating the biomolecule bead-containing tube with a light and detecting a transmitted light or a reflected light from at least a mark bead.

8. A reproducer according to claim 7, reading out the data;

and obtaining information of a DNA or a protein immobilized on the biomolecule beads in the biomolecule bead-containing tube by irradiating the biomolecule beads with a light and observing fluorescence from the biomolecule beads.

9. A reproducer according to claim 7 or 8, obtaining identification information as the data.

10. A reproducer according to claim 9, obtaining arrangement information for the biomolecule beads in the biomolecule bead-containing tube based on the identification information obtained from the biomolecule bead-containing tube.

11. A reproducer according to claim 10, obtaining information of a DNA or a protein immobilized on the biomolecule beads in the biomolecule bead-containing tube based on the arrangement information for the biomolecule beads obtained based on the identification information.

12. A reproducer according to claim 8 or 11, diagnosing a disease from the information of a DNA or a protein obtained based on the identification information.

14. A reproducer according to claim 8, obtaining identification information as the data.

15. A reproducer according to claim 14, obtaining arrangement information for the biomolecule beads in the biomolecule bead-containing tube based on the identification information obtained from the biomolecule bead-containing tube.

16. A reproducer according to claim 14, obtaining information of a DNA or a protein immobilized on the biomolecule beads in the biomolecule bead-containing tube based on the arrangement information for the biomolecule beads obtained based on the identification information.

17. A reproducer according to claim 11, diagnosing a disease from the information of a DNA or a protein obtained based on the identification information.

18. A reproducer according to claim 16, diagnosing a disease from the information of a DNA or a protein obtained based on the identification information.