Hybridization apparatus and method
First Claim
1. A hybridization apparatus for causing hybridization reaction between a sample nucleic acid and a probe nucleic acid by using a nucleic acid chip having the probe nucleic acid bound on the surface of a carrier, which comprises:
- a feeding unit for feeding a liquid to the surface of the carrier, and an observing unit for observing the state of the liquid on the surface of the carrier.
1 Assignment
0 Petitions
Accused Products
Abstract
Hybridization between a sample nucleic acid and a probe nucleic acid with an assay having the probe nucleic acid bound on the surface of a carrier, in which a liquid is fed to the surface of the carrier and the state of the thus-fed liquid is detected. The liquid may, for example, be a hybridization liquid containing the ample nucleic acid, or it may be a washing liquid for flushing byproducts and/or reactants away from the carrier in preparation for detection of hybridization results. Because the state of the liquid is detected, accuracy of hybridization assays is improved, since it becomes possible to detect and avoid difficulties encountered during hybridization and wash processing, such as formation of bubbles and incomplete washing.
-
Citations
21 Claims
-
1. A hybridization apparatus for causing hybridization reaction between a sample nucleic acid and a probe nucleic acid by using a nucleic acid chip having the probe nucleic acid bound on the surface of a carrier, which comprises:
a feeding unit for feeding a liquid to the surface of the carrier, and an observing unit for observing the state of the liquid on the surface of the carrier. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
14. A method for causing hybridization reaction between a sample nucleic acid and a probe nucleic acid by using a nucleic acid chip having the probe nucleic acid bound on the surface of a carrier, which comprises:
feeding a liquid to the surface of the carrier and detecting the existing state of the liquid thus fed to the surface. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21)
Specification