Target molecule attachment to surfaces
First Claim
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1. A method comprising the steps of:
- a. providing a support;
b. disposing a reagent composition on the support, wherein the reagent composition comprises a polymeric backbone having more than one thermochemically amine-reactive or sulfhydryl-reactive groups attached thereto, and wherein the reagent composition is configured and arranged to form covalent bonds with functional groups on a target molecule without use of attracting groups to attract the target molecule to the reagent composition;
c. coupling the reagent composition to the support surface to form a reagent composition-coupled support surface; and
d. disposing at least one target molecule on the reagent composition-coupled support surface, wherein the functional groups of the target molecule forms covalent bonds with the reagent composition.
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Abstract
Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.
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Citations
17 Claims
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1. A method comprising the steps of:
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a. providing a support;
b. disposing a reagent composition on the support, wherein the reagent composition comprises a polymeric backbone having more than one thermochemically amine-reactive or sulfhydryl-reactive groups attached thereto, and wherein the reagent composition is configured and arranged to form covalent bonds with functional groups on a target molecule without use of attracting groups to attract the target molecule to the reagent composition;
c. coupling the reagent composition to the support surface to form a reagent composition-coupled support surface; and
d. disposing at least one target molecule on the reagent composition-coupled support surface, wherein the functional groups of the target molecule forms covalent bonds with the reagent composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification